Liu Jinyao AM, Institute of Molecular Medicine, Shanghai Jiaotong University: Putting a multifunctional coat on probiotics to enhance the therapeutic effect of enteritis

Innovation: Professor Liu Jinyao's research group from the Institute of Molecular Medicine of Shanghai Jiaotong University realized the multifunctional modification of the cell surface by adding other functional molecules during the dopamine polymerization process.

On this basis, the probiotics are coated with a dopamine and chitosan co-deposition coating to make them target enrichment in the colon and enhance the therapeutic effect on enteritis.

Keywords: Advanced Materials, probiotics, surface modification, enteritis, Shanghai Jiaotong University Institute of Molecular Medicine cell therapy, including hematopoietic stem cell reinfusion, chimeric antigen receptor T cell therapy, fecal bacteria transplantation, oral probiotics, etc.
, in many The treatment of this kind of disease shows the advantages and therapeutic effects that conventional drugs can't match, so people are paying more and more attention.

However, the cells themselves are relatively fragile and are susceptible to adverse environmental factors during operation and processing, such as centrifugal force and large temperature fluctuations during operation, gastrointestinal and bile acid damage in the gastrointestinal tract after oral administration, and suffering after transplantation.
Damage to the host immune system and metabolic microenvironment.

These factors can lead to cell damage or death, thereby reducing the effectiveness of disease treatment.

In addition, the process of cell therapy is faced with complex interactions between cells and the diversity of cell targets.
Therefore, there is an urgent need for a strategy to engineer cells that can protect cells at the same time and confer additional exogenous functions to enhance cells.
The effect of therapy.

Recently, Advanced Materials published the "Polymerization-mediated multifunctionalization of living cells for enhanced cell-based therapy" (https://doi.
org/10.
1002/adma.
202007379) paper written by Liu Jinyao's team at the Institute of Molecular Medicine, Shanghai Jiaotong University.

This article introduces a universal dopamine polymerization-mediated multifunctional cell surface modification method.

Polydopamine is a surface coating material inspired by adhesion proteins in mussels, and has good biocompatibility.

With the help of hydrogen bonds and π-π stacking interactions between polydopamine and different functional molecules, as well as Schiff base or Michael addition reactions, other functional molecules can be co-deposited on the cell surface through a one-step process during the polymerization of dopamine to form Multifunctional coating to realize the multifunctional modification of the cell surface.

The human body contains a variety of microbial communities, which play a vital role in maintaining human health.

Many intractable diseases, such as inflammatory bowel disease, diabetes, multiple sclerosis, autism, and certain cancers, are closely related to the composition of the microbial community for their pathogenesis, progression and treatment.

Bacteria can secrete biomolecules such as key proteins and peptides, can effectively regulate the microbial community, and can also respond to a variety of diseases, so they are widely used in the intervention and treatment of different diseases.

Taking oral probiotics as an example, since the introduced probiotics can effectively inhibit intestinal pathogens and regulate intestinal immunity, they have shown great advantages in the treatment of enteritis and other diseases.

However, oral probiotics face the problems of low oral availability and difficulty in effective enrichment at the lesion site caused by factors such as gastric acid and bile acid destruction and colonization resistance.

Based on this multifunctional surface modification method, the author introduced a small amount of chitosan during the polymerization process to put a polydopamine and chitosan co-deposition coating on the surface of the probiotic bacteria.

In the oral drug delivery process, the outer coating can effectively resist the erosion of gastric juice and bile acid, thereby improving the oral bioavailability of probiotics.

In addition, the chitosan in the coat has a certain colonic site targeting ability, which can greatly increase the enrichment of probiotics in the colitis lesions, thereby significantly enhancing the effect of oral probiotic therapy in the treatment of enteritis.

The multifunctional modification method of living cell surface mediated by dopamine polymerization provides an important means for preparing bacteria-mediated intelligent biological preparations, which has very important scientific significance and transformational value.

The above-mentioned research work has been supported by the National Natural Science Foundation of China (21875135), the National Overseas High-Level Talent Introduction Program and other projects.

WILEY paper information: Polymerization-Mediated Multifunctionalization of Living Cells for Enhanced Cell-Based TherapyChao Pan, Juanjuan Li, Weiliang Hou, Sisi Lin, Lu Wang, Yan Pang, Yufeng Wang, Jinyao Liu*Advanced MaterialsDOI: 10.
1002/adma.
202007379 click on the bottom left Corner "Read the original text" to view the original text of the paper.

About Advanced Materials Journal "Advanced Materials" (Advanced Materials) is a well-known authoritative journal in materials science published by Wiley with a history of more than 30 years.

The journal focuses on the frontier development of functional materials in various fields such as chemistry, physics, biology and related interdisciplinary subjects, and has a wide range of influence.

The latest impact factor is 27.
389, and the 2020 SCI journals of the Chinese Academy of Sciences will be divided into the Q1 area of ​​the material science category and the Q1 area of ​​the engineering technology category.

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