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Loop-Mediated Isothermal Amplification for the Detection of Plant Pathogens

 Last Update: 2021-03-12
 Source: Internet
 Author: User

Tags

dna strand calculator

smearing gel electrophoresis

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Loop-mediated isothermal amplification (LAMP) is a technique involving the use of four to six primers (two inner primers, two outer primers, and two loop primers) and the strand displacement activity of
Bacillus subtilis
-derived (
Bst
)
DNA
polymerase. The end result of strand displacement and loop formation and synthesis is the single-temperature amplification of a highly specific fragment from a DNA template at a much greater titre than that obtained with polymerase chain reaction. With LAMP, there are several methods to determine a positive reaction. Presented here are three alternative methods: gel electrophoresis, hydroxynaphthol blue colorimetric dye, and the fluorescent intercalating PicoGreen
�
reagent.

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