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    Home > Biochemistry News > Biotechnology News > Lu Jian task group: the small RNA in fruit flies on the target gene regulation mechanism and action.

    Lu Jian task group: the small RNA in fruit flies on the target gene regulation mechanism and action.

    • Last Update: 2020-08-10
    • Source: Internet
    • Author: User
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    On March 14, 2018, the internationally renowned academic journal Nucleic Acids Research published online in a long essay entitled "Drosophila general lye stun sr. reins reins rein sreire sierosy and via antison sydd and in-the-cellular cyhoeddation response" (DOII: 10.1093 nar//gky189).
    the paper first reported on the regulatory mechanisms and important roles of small RNA (tRNA-derived small RNAs, tsRNA) in fruit flies in target genes and in stress response.
    tsRNA-derived tsRNA is a newly discovered class of small RNA that has been found to be not a random degradation product of tRNA and is associated with processes such as environmental stress response, cell proliferation and cancer, epigenetics, and so on.
    previous studies have shown that tsRNA regulates the overall translation level of cells, but there is no uniform understanding of its mechanisms of action.
    the Lu Jian research team first analyzed small RNA data from fruit flies in public databases and found that most of the tsRNAs in fruit flies are highly conservative, prevalent, and have high abundances, revealing that these tsRNAs may have important biological effects.
    combined with tsRNA cell transfection, mRNA-seq, ribosome profiling, and bi-fluorostine reporting experiments, they found that tsRNA transfection significantly reduced the overall translation level of the cell, and that tsRNA recognized and inhibited the translation of the target gene mRNA through an evolutionaryly conservative complementary sequence.
    their target gene predictions show that tsRNA tends to bind and inhibit mRNA molecules of the main proteins in the cell translation system, explaining how tsRNA inhibits the overall cell translation level through sequence complementary patterns. In addition,
    , experimental results on fruit fly S2 cells in serum hunger indicate that tsRNA is involved in the cell's hunger response process, which is independent of the mTOR pathway but is dependent on the AGO2 protein of the fruit fly.
    based on the above results, the study proposed a model of stress response of "tsRNA serial meters" in fruit fly cells.
    under stress conditions, the cell translation level of an organism usually declines.
    in the "tsRNA serial" model, first the production of tsRNA reduces the abundance of tRNA in the cell and thus the overall translation level of the cell, and second, tsRNA inhibits the translation of mRNA itself of key components of the cell translation system through sequence complementarity specificity, thereby reducing the overall translation level of the cell.
    tsRNA-based chain reactions allow cells to produce a rapid and efficient stress response.
    the study is the first to reveal the critical role of tsRNA-mediated regulatory processes in fruit flies in the energy balance and metabolic adaptability of cells, providing new ideas for a deeper understanding of the mechanisms and functions of tsRNA.
    Lu Jian Researcher is the author of this paper.
    Postdoctoral Student Of the Academy of Sciences Luo Shixuan (now a post-scientist at The Agricultural University of China), He Feng (now a lecturer at Zhejiang University of Medicine), Luo Junjie (now a post scientist at China Agricultural University), Ph.D. student Dou Shengqian, and Wang Wei, ph.D. student of the Joint Center for Life Sciences, are co-authors of the thesis, and Guo Annan, a doctoral student at the Joint Center for Life Sciences. Zhang Hong, a graduate student at the
    School of Health, also provided important help to this study.
    constructive input from Dr. Chen, of the University of Nevada.
    research supported by the Ministry of Science and Technology, the National Natural Science Foundation, the Youth Thousand Samen Program Research Launch Fund, the China Postdoctoral Fund and the Peking University-Tsinghua Joint Center for Life Sciences.
    Source: Bio360.
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