Maintenance of liquid chromatography pumps/columns/detectors (UVs).

related topicsLiquid phase
chromatography
and gas chromatography technology 1.HPLC daily operating conditions:

temperature: 10 to 30 degrees C<;2. Pump maintenance: 1) use the flow phase as far as possible to clean;2) the sand core at the inlet liquid
filter
the head should be cleaned frequently;3) To prevent precipitation when the flow phase is exchanged;4) Avoid blockages or bubbles in the pump.3. Maintenance of the sampler:after each analysis, to repeatedly rinse the inringing port to prevent cross-contamination of the sample.4. Column maintenance: 1) column under no circumstances can collide, bend or strong vibration;2) When the columns and
chromatography
are linked, valve parts or lines must be cleaned;3) pay attention to the degassing of the mobile phase;4) Avoid using high-viscosity solvents as flow phases;5) samples to be purified;6) strictly control the amount of samples;7) After daily analysis work, to clean the sample residue in the sample valve;8) After daily analysis and determination, the column should be cleaned with appropriate solvents;9) If the analysis column is not used for a long time,
be
and closed with organic solvents.5. Detector (UV) maintenance: 1) UV lamp maintenance: before analysis, the column balance is similar, turn on the detector;2) sample pool to be maintained.