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    Home > Biochemistry News > Biotechnology News > Major Breakthrough in Male Infertility Research: Epigenetic Genome of Sperm Defects Becomes Key Cause

    Major Breakthrough in Male Infertility Research: Epigenetic Genome of Sperm Defects Becomes Key Cause

    • Last Update: 2020-05-31
    • Source: Internet
    • Author: User
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    In a recent study published in the international journal DevelopmentAl Cell, scientists from the University of Pennsylvania used new genome-wide DNA sequencing tools to illustrate the exact genetic unknowns of these stranded histones, as well as the key genes that regulate themIn the paper, the researchers used mouse models of the Gcn5 gene mutation to study, which helps them closely track sperm defects from early stages of sperm development to fertilization, a step that could help researchers gain a deeper understanding of the mechanisms in which male infertility occurs, and also illustrate how epigenetic mutations are transferred from male reproductive cells to embryosepigenetic factor is a special factor that does not encode in DNA and can affect the genetic properties of the organism and plays a key role in sperm and egg formation; researcher Shelley LFor men with unexplained infertility, everything is normal in the eyes of doctors, including sperm counts, exercise, and so on, but it does have fertility problems, drBerger said, and one explanation for this problem is that the unknown error in histones is that it affects sperm and its early development, and now researchers have developed a new model that allows researchers to clearly observe what happens if the hetin protein in sperm is not properly removed and how it affects the embryohealthy sperm loses 90%-95% of histones (the main protein in chromatin, which is responsible for wrapping DNA and turning on or off gene expression), and then replaces histins, a small protein that correctly wraps DNA into tiny sperm, given the role of histones in infertility and embryonic development, so that they can be studied laterFor the study, researchers used a new technique called MNase sequencing, which uses enzyme-promoting reactions to determine the exact location of stranded histones on important gene startersOther studies using the same method have found that histones are present in DNA repetition sequences and are placed in so-called "genetic deserts", where histones play a less regulating roleresearcher Luense said there is some controversy among researchers trying to understand these differential data, and in this study we found that the model previously described was correct, that we found histones on genes that are important for embryonic development, and that histones were located on repetitive components that really needed to be closed to prevent these gene regions from being expressed in the embryoThe researchers then used a more precise sequencing technique, ATAC sequencing, to track the histin wave waves at special sites in the genome of sperm in mice early and late stages, which identifies open and closed areas of the genome and then cuts and labels DNA for sequencingWhen studying mouse models of the Gcn5 gene mutation, the researchers found that these mice had lower fertility levels and that the location of histones trapped in normal mouse sperm was related to the location of histones in early embryos, supporting the hypothesis that progenitor histone metastases would be passed on to the next generation;researcher Berger says that the burden of IVF and other assisted reproductive technologies now falls on women, and sometimes male factors are crucialImagine if we could use epigenetic therapy to change the levels of histones and sperm proteins in men's sperm before the embryo stakes? This may be the next step for researchers to exploreGiven the mechanism that there are a number of epigenetic drugs that can treat cancer and other diseases, the use of these drugs to treat sperm to increase the "expulsion" of histones may be worth later in-depth studyThe limitations currently faced by scientists in human embryo research can lead to a lack of research on infertility and the effects of the parent's epigenetic genome on embryonic development, which may underscore the importance of such research, and finally, researcher Luense said, there are many different factors that alter the epigenome of sperm, including diet, drugs, alcohol, and now we need to further study the mechanisms and factors that influence the development of these subjects(Bio ValleyBioon.com)Origin: Lacey J Luense, Greg Donahue, Enrique Lin-Shiao, et al Gcn5-Mediated Histone Acetylation Nucles Nucleios In Spermios, Cellal (2019) doi:10.1016/j.devcel.2019.024.2019.024
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