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    Home > Chemicals Industry > Chemical Technology > Maximum allowable residue analysis technology of nitrofuran metabolites-determination method (3)

    Maximum allowable residue analysis technology of nitrofuran metabolites-determination method (3)

    • Last Update: 2021-09-20
    • Source: Internet
    • Author: User
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    (4) Immunoanalysis (IA)

    The immunological analysis method is a determination method established based on the antigen-antibody specific reaction.
    It has many advantages such as simplicity, speed, large sample size, high sensitivity, and strong specificity.
    The disadvantage is that the false positive rate is high
    .

    1) Enzyme linked immunosorbent assay (ELISA)

    Cooper et al.
    first used the AOZ derivative CPAOZ to connect with HAS to prepare a complete immune antigen, and successfully obtained polyclonal antibodies with high sensitivity and strong specificity, and there was almost no cross-reaction with NFs metabolites and other compounds except AOZ
    .


    The IC 50 value is 0.


    Wang Qinhui and others used an indirect competitive ELISA method to determine the nitrofurantoin metabolite AHD in pork
    .


    The conjugated antigen is pre-coated on the microwell strip of the microtiter plate, and the residue AHD in the sample is derivatized and competes with the pre-coated coupled antigen on the microwell strip for the anti-furantoin metabolite derivative antibody, and the enzyme label is added After the secondary antibody, the TMB substrate is used for color development, and the absorbance value of the sample is negatively correlated with the content of the AHD metabolites contained in the residue.


    Vass et al.
    applied molecular simulation to study the synthesis of antigens, obtained polyclonal antibodies of the nitrofural metabolite SEM, and established a direct competition ELISA method
    .


    The obtained SEM polyclonal IC 50 is 0.


    2) Colloidal gold immunochromatography (immune colloidal gold technique, GICT)

    Pan Xinhong et al.
    immunized mice with AMOZ coupled with bovine serum albumin (BSA) to prepare hybridoma cells, obtained purified anti-AMOZ monoclonal antibodies, and prepared colloidal gold test strips
    .


    The semi-quantitative method of colloidal gold test paper was used to directly determine the content of furanolide metabolite AMOZ in fish meat


    3) Fluorescence immunoassay (fluoroimmunoassay, FIA)

    Shen Yudong et al.
    established for the first time a fluorescence polarization immunoassay (FPIA) method for the SEM of the metabolite of nitrofurazone
    .


    By designing and synthesizing a new SEM hapten CEPSEM, coupled with carrier protein, immunized New Zealand white rabbits to prepare polyclonal antibodies with high affinity and good specificity


    4) Immunosensor

    Yang et al.
    established a fast and reliable electrochemical method to detect AOZ based on the traditional label-free electrochemical impedance titration immunosensor
    .


    The AOZ monoclonal antibody (AOZ-McAb) is coated on the gold electrode by the stable acylamino ester intermediate produced by EDC and NHS, which can condense the antibody on the self-assembled monolayer (SAM)


    5) Biochip

    O'Mahony et al.
    applied chemiluminescence-based biochip array sensing technology to the screening of NFs residues in honey
    .


    Through this composite technology, four NFs metabolites can be detected simultaneously


    Related Links: Analytical Technology for Maximum Allowable Residues of Nitrofuran Metabolites-Determination Method (2)

     

     

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