Measurement of crude fat content - Soshi pumping.

, purpose
fat is widely found in the seeds and fruits of many plants, the determination of fat content, can be used as an indicator to identify its quality. There are many methods for determining fat content, such as pumping, acid hydrolysing, specific gravity, refraction, electrometric and MRI. At present, the method of pumping is widely used at home and abroad, among which Soxhlet extractor method is recognized as a classic method and the standard method of the preferred method of grain and oil division in China. Through the study of this experiment, we can master the principle and operation method of measuring the crude fat content by the Soshi method.
II, the original
this experiment uses the residual method of the Soshi extraction method, that is, with a low boiling point
organic
solvent (ether or petroleum ether) reflow extraction, remove the crude fat from the sample, to the sample and residue weight difference, calculate the crude fat content. Because the extract of organic solvents, in addition to fat, but also more or less contain free fatty acids, sterols, phospholipids, waxes and pigments and other lipid-like substances, so the results of the extraction method can only be crude fat.
III, experimental materials, main instruments and
reagents
1, experimental materials
oil crop seeds, medium speed
filter paper
2, instruments:
(1) Soshi fat Drawer (Figure 1) or YG-II. type oil partser
(2)
drying
(diameter 15 to 18cm, color-changing silicone)
(3) Stainless steel tweezers (20cm long)
(4)
cultured
dishes
(5) Analysis
balance
(sense 0.001g)
6) Weighing bottle
(7)
thermostat
water bath
(8)
oven(9) sample sieve (60
).