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Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt

 Last Update: 2020-12-03
 Source: Internet
 Author: User

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This chapter describes an indirect approach to measure PTEN’s lipid phosphatase activity
in vivo
. PTEN counteracts phosphatidylinositol 3-kinase action in dephosphorylating 3-phosphorylated phosphoinositides. Therefore, PtdIns(3,4,5)P
3
-dependent activation and phosphorylation of the survival kinase Akt can be used as readout for cellular PTEN activity. Here we have outlined a detailed procedure employing a phosphoserine-specific anti-Akt antibody to examine the content of phosphorylated Akt by immunofluorescence and its dependence on PTEN activity.

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