MED23 subkyls are involved in regulating pigmentation and DNA damage repair in pigment cells.

Recently, the research results of the Wang Gang Research Group of the Institute of Biochemistry and Cell Biology of the Shanghai Institute of Life Sciences of the Chinese Academy of Sciences were published online in Cell Reports under the title Mediator MED23 Links Pigmentation and DNA Repair Through The Transcription Factor MITF.
the study revealed that transcription of the intermediary complex MED23 sub-base was involved in regulating pigmentation and DNA damage repair processes in pigment cells.
hair, skin, eyes, etc. are mostly determined by melanin in melanoma cells, melanin synthesis process is very complex, by a variety of signaling paths.
When the skin is stimulated by ultraviolet light (UV) in the sun, melanin cells produce and secrete melanin-to-skin cells that absorb ultraviolet light and reduce DNA damage, thus protecting the stability of the genome.
addition, UV stimulation causes DNA damage and nucleotide removal repair (Nucleotide excision repair(NER), and the cytochrome process is closely related to DNA damage repair, which is one of the hot topics in this field.
mammalian transcription media complex is a multiprotein complex of about thirty evolutionaryly conservative proteins.
When stimulated by environmental or developmental signals, different transcription factors interact with specific sub-substations in the intermediary complex, which in turn recruits generic transcription machines such as universal transcription factors and RNA polymerase II to start the transcription of genes.
but the function and function of transcription intermediary complex in pigment synthesis and DNA damage repair are not clear.
the researchers found that after the mouse melanoma cell B16F10 knocked down the transcription intermediary complex Med23 sub-base, the cells turned white, the melanin content decreased significantly, and the melanin subsethics of synthetic melanin decreased.
addition, after knocking down Med23 in zebrafish, the melanin content of zebrafish decreased and the expression of important genes in pigment synthesis decreased.
results of in vitro experiments show that MED23 regulates pigment synthesis process.
the absence of Med23, the amount of DNA damage caused by UV was reduced, and the expression of NER factors and the amount of their binding to chromosin increased significantly, indicating that MED23 regulates the DNA damage repair process.
further research has found that MED23 is using the positive regulation of the expression of the transcription factor Mitf (Microphthalmia transcription factor) to evenly link pigment synthesis and DNA damage repair processes.
by ChIP-seq, CRISPR-Cas9, and the reporting gene system, MED23 regulates Mitf's gene expression by regulating the activity of Mitf far-end enhancers.
the study sheds light on the molecular regulation mechanism of transcriptional media in pigment synthesis and DNA damage repair, and provides new ideas for skin-related disease intervention.
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