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Medicago truncatula Transformation Using Leaf Explants

 Last Update: 2021-01-19
 Source: Internet
 Author: User

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Legumes have long been recalcitrant to efficient
Agrobacterium tumefaciens
-mediated transformation. The choice and use of model legume plants (
Medicago truncatula
and
Lotus japonicus
) for molecular studies has triggered extensive studies devoted to the development of efficient
Agrobacterium
-mediated transformation protocols for these two plants. In
M. truncatula
, transformation protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on
Agrobacterium
-mediated transformation of
M. truncatula
followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for
M. truncatula
R108-1 (c3).

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