Melon seed gold saponins

Melon seed gold saponins

Polygalasaponin F

【Structural formula】

[Molecular formula and molecular weight] C ₅₃ H ₈₆ O ₂₃ ; 1091.
24

[Source] The dry whole plant of Polygala japonica Houtt
.

【Properties】White powder
.

This product is soluble in methanol and water
.

Melting point: 232～233℃
.

【Purity Check】

TLC

1.
Thin layer plate silica gel G

Developing solvent n-butanol - acetic acid - water (4: 1: 5) upper solution

Check the color development of 10% sulfuric acid ethanol solution, check under sunlight

2.
Thin layer plate silica gel G

Developing solvent chloroform - methanol - water (75:35:4)

Check the color development of 10% sulfuric acid ethanol solution, check under sunlight

High performance liquid chromatography

Chromatographic column Agilent C ₁₈ , 5um (4.
6mm×250mm)

Mobile phase acetonitrile - water (28:72), 1.
0ml / min

Evaporative light scattering detection, drift tube temperature 105℃, carrier gas flow rate 2.
8ml/min

【Structural identification】203
.

3387, 2942, 1751, 1637, 1460, 1386, 1257, 1159, 1077, 1046, 893, 817
.

ESI-MS m/z: 1089 [MH] ^-
.

¹ H-NMR (C ₅ D ₅ N) δ: 0.
78 (3H, s, H-30), 0.
81 (3H, s, H-29), 1.
14 (3H, s, H-26), 1.
19 (3H, s, H-27), 1.
26 (3H, s, H-24), 1.
52 (3H, s, H-25), 1.
81 (3H, s, J=6.
0Hz, H-6"), 5.
43 (1H, brs, H-12), 5.
13 (1H, d, J=7.
8Hz, H-1'), 6.
17 (1H, d, J=8.
4Hz, H-1"), 6.
48 (1H, d, brs, H -1"), 5.
05(1H, overlapped, H-1")
.

¹³ C-NMR (C ₅ D ₅ N) δ: 44.
3(C-1), 70.
6(C-2), 83.
1(C-3), 42.
5(C-4), 47.
9(C-5), 18.
2( C-6), 32.
4(C-7), 40.
1(C-8), 48.
6(C-9), 37.
1(C-10), 24.
1(C-11), 122.
9(C-12), 144.
2(C -13), 42.
8(C-14), 28.
6(C-15), 23.
4(C-16), 47.
2(C-17), 42.
1(C-18), 46.
4(C-19), 30.
7(C- 20), 34.
0(C-21), 33.
2(C-22), 65.
6(C-23), 15.
1(C-24), 17.
4(C-25), 17.
6(C-26), 26.
1(C-27) ), 176.
5(C-28), 33.
1(C-29), 23.
8(C-30), 105.
7(C-1'), 75.
5(C-2'), 78.
6(C-3'), 71.
6(C -4'), 78.
3(C-5'), 62.
7(C-6'), 94.
8(C-1"), 76.
6(C-2"), 79.
6(C-3"), 71.
3(C-4 "), 79.
0(C-5"), 62.
1(C-6"), 101.
5(C-1"'), 71.
9(C-2"'), 72.
7(C-3'"), 85.
6(C- 4"'), 68.
3(C-5'"), 18.
7(C-6"'), 107.
8(C-1""), 76.
3(C-2""), 78.
8(C-3""), 71.
0(C-4""), 67.
5(C-5"")
.

【Storage】Keep tightly closed
.