 Home > Biochemistry News > Biotechnology News > Membrane Insertion of Small Proteins

Membrane Insertion of Small Proteins

 Last Update: 2021-02-27
 Source: Internet
 Author: User

Tags

purified protein derivative

small size printer

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Proteins that are less than 10 kDa in size are easily purified under denaturing conditions and can often be refolded by removal of the denaturing agents. The purified small membrane proteins are competent for membrane insertion when the denaturing agent is diluted out and a membranous system like liposomes or proteoliposomes is added. This system allows the characterization of the membrane insertion process at the molecular level. The insertion of the protein into proteoliposomes can be followed by protease digestion and Western blot analysis. Only if the antigenic region of the protein has translocated into the lumen of the proteoliposome it is protected from the protease. When combining this approach with fluorophores that are placed within the membrane protein, membrane insertion can also be followed by fluorescence correlation spectroscopy.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

L-Ascorbate-2-Monophosphate Feed Grade

CAS No.: 109620-90-8

Feed Grade

Potassium Sorbate from reliable manufacturer

CAS No.: 590-00-1

Food Grade

Doxycycline Hydrochloride

CAS No.: 10592-13-9

Pharmaceutical Grade

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.