 Home > Biochemistry News > Biotechnology News > Methods in Functional Proteomics: Two-Dimensional Polyacrylamide Gel Electrophoresis With Immobilized pH Gradients, In-Gel Digestion, and Identification of Proteins by Mass Spectrometry

Methods in Functional Proteomics: Two-Dimensional Polyacrylamide Gel Electrophoresis With Immobilized pH Gradients, In-Gel Digestion, and Identification of Proteins by Mass Spectrometry

 Last Update: 2020-12-27
 Source: Internet
 Author: User

Tags

proteins act

diclofenac gel 1

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

The recent combination of several technology platforms has revolutionized the analysis of expressed cellular proteins (proteomics). Large-scale separation and visualization of proteins is accomplished using two-dimensional gel electrophoresis (2-DE). Proteins are separated first by isoelectric focusing (IEF) using immobilized pH gradient (IPG) strips. Following charge separation, proteins are further resolved by molecular mass using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (
SDS
-PAGE), as shown in Fig. 1.Fig. 1.
2-DE gel pattern of WM35 (melanoma) cell line.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

L-Ascorbate-2-Monophosphate Feed Grade

CAS No.: 109620-90-8

Feed Grade

Potassium Sorbate from reliable manufacturer

CAS No.: 590-00-1

Food Grade

Doxycycline Hydrochloride

CAS No.: 10592-13-9

Pharmaceutical Grade

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.