Methyl esterification: New nickel modification seislicing can be efficient lysing with NAD-synthetic path mutants (ao-1 and qs-1)

NAD (Nickamide adenine dinucleotide) as an electron delivery carrier (coenzyme) involved in a large number of redox reactions, for the vast number of researchers are well known. the discovery of NAD-consuming enzymes
has once again aroused the enthusiasm of researchers for their research on remedial synthesis pathways.
differs from the two-step remediation synthesis of NAD in mammals, which is the four-step Preiss-Handler pathway in terrestrial plants, and where there are a variety of derivatives of nicotine (nicotine, NA) specifically present in plants (glycosylation, methylation, etc.).
so far, there have been few reports on the molecular mechanism and physiological function of NA derivatives in plant metabolism.
an earlier study by the WangDong Research Group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, NA's O-bit glycosylation modification may protect plant cells from the toxic effects of NA overaccumulation during seed germination, and NAOGT activity is only gradually acquired during the evolution of the cruciude plant, and the acquisition of NAOGT activity provides a selection advantage for the plant adaptation environment (Li et al.Plant). Cell, 2015); NA's N-methyl transferase (NANMT) is another form of plant detoxifying NA, and the acquisition of NANMT activity may be an important reason for the retention of the Preiss-Handler pathway in terrestrial plant genomes (Li et al., Plant Physiol., 2017).
in the latest research work, the Kingdom Dong research team found a new type of nickel acid modification, meNA, which can be efficiently complementary nad from the head synthesis path mutants (ao-1 and qs-1), suggesting that MeNA can be transported over long distances between different tissues of plants and participate in NAD biosynthesis. the
study group further cloned the genes responsible for NA methylation and MeNA demethylation, using relevant genetically modified materials combined with stable isotope markers and chemical analysis, showed that NAD was redistributed between coerced and non-coergent tissues through NA reversible methylation modification, thereby improving plant adaptability to different stressed environments.
the results provide a basis for further study of how the Preiss-Handler approach provides the advantages of choice for terrestrial plants in evolution.
related research results published in Molecular Plant, Wang Dong Research Group, Dr. Wu Ranran, Zhang Fengxia as co-first author.
the research was funded by the National Natural Science Foundation of China, the National Key Basic Research and Development Program and the National Key Laboratory of Plant Genomics.
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