Micro-core detection technology

, the purpose of the experimentLearn to use the knowledge learned, carry out independent experimental design, understand the various types and principles of chromosomal amortation, master the use of micro-nucleoelectrical detection technology to monitor environmental pollution in general.
, the principle of experimentMicronucleus, also known as a satellite nucleus, is an abnormal structure in the cells of the ethynuclearius, a manifestation of chromosomal amortism in interstitial cells. Microkernels are often produced by various physical and chemical factors, such as radiation and chemical agents, that act on dividing cells. During the intercellular period, the micro-core is circular or elliptical, free from the main core, the size should be less than 1/3 of the main core. The recactive rate of the micro nucleation and the properties of the cellular chemical reaction are the same as that of the main nucleo, and have the ability
synthesize
DNA. It is generally believed that the micro nuclea is produced by chromosomal fragments that lose their silk particles in the later stages of silk division. Experiments have shown that an entire chromosome or several chromosomes can also form microkernels. These fragments or chromosomes lag behind in the process of division and fail to enter the primary nucleosphere at the end of the split, forming a nucle block outside the main nucleosphere. When the subcells enter the next interstate, they are concentrated into small nucleuts outside the main nucleo, which form microkernels.
has been shown that the size of the micro-nucleogram is positively related to the dose of the action factor or the cumulative effect of radiation, as is the case with chromosomal distortion. So many people think that simple periodic micro-nucleus counts can be used instead of complex midterm distortion chromosomal counts. Due to the synthesis of a large number of new
compounds
, the application of atomic energy, the discharge of various types of industrial waste, etc. there is the possibility of polluting the environment, in order to understand the potential genetic harm of these factors to the body, a highly sensitive, technically simple test system is needed to monitor environmental changes. Only the test system of the enernel class can directly speculate on the genetic harm of mutable substances to humans or other advanced organisms, in which case micro-core testing is an ideal method. At present, many departments at home and abroad have used micro-nuclear testing for radiation damage, radiation protection, chemical mutagents, new drug tests, food additives safety evaluation, as well as chromosomal genetic diseases and pre-cancer diagnosis and other aspects.
, the experimental material
bean (vicia faba, 2n x 12) dried seeds.
, experimental instruments and appliances,Microscope
, manual counter, tweezers, slide, cover slide,
beech
, porcelain plate,
culture
box, analysis
balance
, water bath pot, alcohol lamp, scissors, anatomical needle,
filter paper
, pencil, label paper, glue, etc.
, pharmaceutical and
reagentsaccording to the requirements of student test design, the corresponding drugs and reagents.
, the experimental steps(1) Experimental design
students may choose different methods to design their own mutation treatment of materials, and the test design will be handed over to the guidance teacher for examination and test operation.
(2) Experiment implementation
students to carry out experiments independently according to experimental design. and write an experimental report.
, the experimentalWhat kind of splitting images might occur in the middle of division of the root tip cells that produce the micro nucleation?
。