Microbial Laboratory Workflow

In order to prevent cross-contamination and protect the health of workers, the microbiological laboratory is divided into four zones: pollution zone, semi-polluting area, sterile area, clean area, the following is the working system of each district, pollution area(i) laboratory staff should wear work clothes and wear ingenuity, hats, gloves and shoe covers when entering the operating area, in strict accordance with operating procedures(ii) after working every morning, after working every day, with ultraviolet light exposure of 60 min, disinfection of the entire operating area ;(3) The district can only carry out: the reception of clinical specimens, preservation, specimen inoculation, culture, pre-test treatment of their strains, the preparation of bacteria suspension, staining specimen examination, non-on-machine identification and drug sensitivity test operations and other dangerous operations, other operations shall not be carried out in this area(Note: Specimens must be inoculated and smeared in biosafety cabinets, and direct smears, gram-dyed and acid-dyed tablets must be exposed to 30 min of UV rays from biosafety cabinets before they can be taken out for staining observation.)(iv) Contaminant treatment: the operating area is equipped with disinfection tanks to deal with contaminated items such as liver scantilylist tablets contaminated with bacteriaThe remaining specimens and used concentrated sites such as sterile plates are safely placed, disinfected and sterilized before washing or discarding(v) the operating area must be cleaned or disinfected immediately after the end of workThe operating area is grounded with a special mop todrag 1 time a day and scrubbed with disinfectant once a weekDisinfect the work surface with disinfectant after the afternoon work to ensure the safe and clean working environment(vi) Non-staff members of this laboratory shall not enter without permissionsecond and semi-polluting areas(i) laboratory staff in this area wear special work clothes and wear ingenuity masks, hats, gloves and shoe covers, in strict accordance with operating procedures(ii) after working every morning, after working every day, with ultraviolet light exposure of 60 min, disinfection of the entire operating area ;(iii) This area can only be carried out: microbial on-machine identification and drug sensitivity analysis, blood culture instrument germination culture, related experimental records and reports written, printed;(iv) the operating area must be cleaned or disinfected immediately after the work is completed The operating area is grounded with a special mop todrag 1 time a day and scrubbed with disinfectant once a week Disinfect the work surface with disinfectant after the afternoon work to ensure the safe and clean working environment (v) Non-staff members of this laboratory shall not enter without permission 3, sterile area (i) laboratory staff in this area should wear special work clothes and wear ingenuity masks, hats, gloves and shoe covers, in strict accordance with the operating procedures (ii) the sterile chamber must be disinfected with ULTRAviolet light for 30 min before use, the countertop is cleaned after operation and then 30 min is disinfected with ultraviolet light Regularly fumigate with lactic acid or formaldehyde and disinfect thoroughly (iii) The district can only carry out the preparation of storage reagents, reagent assembly, culture tube and plate preparation and other sterile operations, can not carry out the operation of bacteria specimens (4) The sterile room is accessible only to the operator and is strictly closed during operation IV, clean area (i) cleaning area for the preparation of the medium and reagent storage, documentation and staff rest and staff work preparation (ii) the cleaning area is prohibited from bringing in bacterial test specimens disinfect the worksurface with disinfectant every day to ensure the safe and clean rest environment