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Extracellular Vesicles (EVs) are vesicle-like bodies with a double-layer membrane structure that are shed from the cell membrane or secreted by the cell.
The mRNA content of extracellular vesicles is extremely low and the detection procedure is complicated.
This technical study established an ultra-sensitive, fast, and enzyme-free DTTA method for in situ detection of PSA mRNA in extracellular vesicles, using prostate-specific antigen genes as a surrogate organism when identifying patients with prostate cancer and benign prostatic hyperplasia The markers have better diagnostic accuracy than serum prostate-specific antigen protein, which may greatly expand the application of liquid biopsy supported by DNA nanostructures