Molecular biology is commonly used in reagent configurations.

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Molecular Biology
the preparation of commonly used storage fluids
1, 30% acrylamide solution
(preparation method) 29g acrylamide and 1g N, N'-methylene diacrylamide dissolved in a total volume of 60ml of water.
heating
to 37 degrees C dissolved, recharging water to the final volume of 100 ml. filter (0.45 m aperture)
filtration
sterilization, confirmed that the solution pH should not be greater than 7.0, stored at room temperature in a brown bottle.
acrylamide is highly neurotoxic and can be absorbed through the skin, and its effect is cumulative. Wear protective gloves and masks when weighing acrylamide
methylene
acrylamide. Polyacrylamide may be considered non-toxic, but it should also be operated with caution as it may also contain small amounts of non-polymerized material.
some lower-priced acrylamides and diacrylamides usually contain metal ions, add about 0.2 volumes of single-bed mixed resin (MB-1 Mallinckrodt) to acrylamide storage fluids, stir overnight, and then filter them with Whatman 1
filter paper
for purification.
during storage, acrylamide and diacrylamide slowly

into acrylamide and biacrylic acid.
2, 40% acrylamide
(preparation method) 380g acrylamide (
DNA
sequencing stage) and 20g N, N'-methylene diacrylamide dissolved in a total volume of 600ml distilled water. Continue to be treated according to the above preparation of 30% acrylamide solution, but after heating dissolved should be filled with distilled water to the final volume of 1L.
note: See the above preparation of 30% acrylamide instructions, 40% acrylamide solution for DNA sequence determination.
3, line mycobacteria D solution
(preparation method) 20 mg of line mycobacteria D dissolved in 4 ml of 100% ethanol, 1:10 diluted storage fluid, with 100% ethanol as a blank control reading OD440 value. The molar digestion coefficient of the pure product in the aqueous solution is 21,900, so the absorbent value of 1mg/ml of line mycobacterium D solution at 440nm is 0.182, and the storage fluid of line mycobacterium D should be stored in
test tube
with foil and stored at -20 degrees C.
Note: Line mycosin D is a teratogen and carcinogen, the solution must be prepared with gloves and in the ventilation cabinet operation, not in the open on the experimental table, beware of inhaling powder or let it touch the eyes or skin.
D products provided by pharmaceutical companies for therapeutic purposes often contain additives such as sugar or salt. Such products can be used to inhibit their own guidance as long as the concentration of linen D is determined by measuring the light absorption of the storage fluid at a wavelength of 440nm.
4, 0.1mol/L adenosine triphosphate (ATP) solution
(preparation method) dissolves 60mg ATP in 0.8ml water, adjusts to pH to 7.0 with 0.1mol/L NaOH, distilled Water capacity of 1 ml, divided into small parts stored in -70 degrees C
5, 10mol / L acetyl solution
"preparation method" to dissolve 770g acetyl in 800ml of water, water content to 1L after filtration and sterilization.
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