Molecular Cancer recently published an article on the application of Arraystar Circular RNA chip to study liver cancer

Recently, Academician Fan Jia, Dean of Zhongshan Hospital of Fudan University, published a research paper on CircMEMO1 modulates the promoter methylation and expression of TCF21 to regulate hepatocellular carcinoma progression and sorafenib treatment sensitivity in the field of liver cancer

Corresponding author:

Research background

Research ideas

Functional study

Mechanism research and

Technical route

The results show that

Figure 1.

Figure 2: CircMEMO1 inhibits HCC cell proliferation and invasion in vitro and in vivo

Figure 3: CircMEMO1 regulates the level of TET1/5hmC axis by smearing MiR-106b-5p in HCC cells

Upper left: Schematic diagram of the binding sites of circMEMO1 and miR-106b-5p, and diagrams of the binding sites of miR-106b-5p with TET1 and TET2

Research significance:
In conclusion, in this study, the authors found that CircMEMO1 is an important tumor suppressor in liver cancer metastasis and stem cell differentiation.
It plays a role through the miR-106b-5p/TET1/5hmC/TCF21 axis and the EMT process
.
As a key epigenetic modifier, CircMEMO1 can also regulate the sensitivity of liver cancer cells to sorafenib treatment
.

Article link:

https://molecular-cancer.
biomedcentral.
com/articles/10.
1186/s12943-021-01361-3

Kangcheng BiologyRelated technical services that Digital Spectrum Biology can provide

l CircRNA expression profile detection- Arraystar circRNA chip:

Design specific probes for the CircRNA reverse splicing site to accurately detect circRNA , which is more suitable for circRNA expression profile analysis
than sequencing .
Assist customers to publish more than 350 SCI articles;

l circRNA qRT-PCR:

The technology is mature, circRNA reverse splicing site design primer, specific detection target circRNA
.

l circRNA and protein interaction (circRNA ChIRP-MS)

CircRNA can be used as a protein sponge or a backbone for protein binding, affecting protein function
.
 circRNA ChIRP-MS by treatment with biotin -labeled RNA anti-sense with the crosslinked sample hybridization, by immobilized avidin to biotin for the Pull-Down , to obtain RNA binding protein ( RBP ) and identified by mass spectrometry target circRNA protein binding
.

Exosomics:

Exosomes isolated, identified exosomes, the exosomes transfer recording exosomes genomics and proteomics

More circRNA research technology platforms:

l circRNA m6A detection- Arraystar circRNA epitranscriptome chip:

Detection of m6A modified circRNA ; quantitative modification percentage and modification changes; total RNA requirement is as low as 3ug
.

l circRNA m6A methylation PCR detection:

( 1 ) Verify or detect a certain m6A-   modified circRNA : m6A antibody enriches the m6A- modified circRNA + circRNA reverse splicing site design primers to ensure accurate detection of m6A-circRNA

( 2 ) determined to contain a potential m6ACA site circRNA whether a real occurrence m6A modification: RNase R & lt go linear RNA + MazF enzyme treatment  + m6ACA site PCR detection .