Molecular Cell: Proteome Plasticity in Response to Continuous Temperature Rising

Ordinary yeast can adapt and thrive by changing the shape, position and function of its protein to cope with the long-term temperature rise

In the wild, temperature is an unstable parameter.

He said: "This is a crucial issue, because the rise in temperature caused by climate change and global warming will affect the offspring of most species currently living on the earth

In this study, Buck researchers tracked and compared yeast grown at room temperature with cells grown at 95 degrees Fahrenheit (35 degrees Celsius) for more than 15 generations

Through unbiased imaging screening and machine learning-based image analysis, the scientists analyzed millions of cells in the entire yeast proteome and found that after the cells adapted to higher temperatures, hundreds of proteins changed their expression Patterns, including richness and subcellular location

"The most exciting and unexpected changes occur at the submolecular level of proteins," Zhou said.

This discovery comes from a new proteomics-structure screening pipeline developed by Zhou and his colleagues, which allows them to identify many proteins that adopt another shape or conformation after the yeast has adapted to a new environment

By examining the interactions between proteins and related molecular functions, the researchers found that Fet3p produced at different temperatures has different functions in different cell compartments

Although an evolutionary coding strategy was discovered that allowed yeast to adapt to different temperatures, Zhou pointed out that flexibility cannot be assumed

Zhou will continue to in-depth study the molecular details of cell internal changes during long-term temperature changes, and plan to incorporate simple animals into the exploration of protein plasticity

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Proteome plasticity in response to persistent environmental change

DOI: 10.