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    Home > Biochemistry News > Biotechnology News > Molecular Cell: Proteome Plasticity in Response to Continuous Temperature Rising

    Molecular Cell: Proteome Plasticity in Response to Continuous Temperature Rising

    • Last Update: 2021-07-27
    • Source: Internet
    • Author: User
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    Ordinary yeast can adapt and thrive by changing the shape, position and function of its protein to cope with the long-term temperature rise
    .
    These surprising discoveries indicate the unrecognized plasticity of proteins at the molecular and conformational level, and bring the power of molecular biology to biological responses to climate change

    .
    The results of the collaboration between the Zhou laboratory of the Buck Institute and the Si laboratory of the Stowers Institute were published in the journal Molecular Cell

    .

    In the wild, temperature is an unstable parameter.
    By changing the stability of protein and the speed of metabolism, it affects almost all aspects of life

    .
    Dr.
    Chuankai Zhou, a researcher at the Booker Institute, is the lead scientist of the study.
    He said that previous studies have provided extensive knowledge about how acute, short-term temperature rises can cause protein misfolding and reveal how cells can upregulate molecular chaperones.
    And other stress response proteins to meet these challenges, refolding/degrading these misfolded proteins to help unprepared cells survive the sudden changes in the environment

    .
    However, Zhou said that when temperature rises become a long-term challenge, it is largely unknown whether cells will continue the misfolding-refolding/degradation cycle of this protein

    .

    He said: "This is a crucial issue, because the rise in temperature caused by climate change and global warming will affect the offspring of most species currently living on the earth
    .
    " "Understand how and whether these organisms are at the molecular level.
    China is prepared for this long-term global warming, which is vital to our solution to the future of the ecosystem

    .
    "

    In this study, Buck researchers tracked and compared yeast grown at room temperature with cells grown at 95 degrees Fahrenheit (35 degrees Celsius) for more than 15 generations
    .
    Higher temperatures initially led to well-documented stress responses, such as short-term temperature increases (or heat shock), including protein aggregation and increased expression of protective sexual partners

    .
    Researchers found that after yeast has grown for several generations at high temperatures, the cells returned to normal and the growth rate gradually accelerated

    .
    After 15 generations, protein aggregation disappeared, and many acute stress regulators returned to baseline expression levels

    .
    Whole-genome sequencing did not find genetic mutations

    .
    Zhou said that to some extent, yeast has adapted to the temperature challenge

    .

    Through unbiased imaging screening and machine learning-based image analysis, the scientists analyzed millions of cells in the entire yeast proteome and found that after the cells adapted to higher temperatures, hundreds of proteins changed their expression Patterns, including richness and subcellular location
    .
    "Interestingly, after yeast adapts to the new environment, the expression of proteins that are prone to misfolding during acute stress decreases," Zhou said

    .
    "This shows that under continuous temperature challenges, a possible strategy to avoid misfolding/refolding cycles is to reduce the load of heatable proteins

    .
    " Zhou said that subcellular localization is a determinant of protein function

    .
    These proteins change their subcellular distribution under continuous temperature changes to protect themselves from thermal instability or perform new functions, as compensation for the reduction of other heat-labile proteins, or both

    .

    "The most exciting and unexpected changes occur at the submolecular level of proteins," Zhou said.
    "Once yeasts'realize' that heat stress is long-term, they change a lot

    .
    Some of their proteins change their conformations.
    (Shape)

    .
    The current paradigm of gene-protein function research is based on the belief that proteins have a final structure

    .
    Our research shows that this is not the case at least for some proteins that respond to temperature changes

    .
    "

    This discovery comes from a new proteomics-structure screening pipeline developed by Zhou and his colleagues, which allows them to identify many proteins that adopt another shape or conformation after the yeast has adapted to a new environment
    .
    Importantly, the conformational changes of these proteins are not caused by genetic mutations, and most of them do not result in post-translational modifications

    .
    Taking Fet3p (a glycoprotein containing multiple copper) as an example, the researchers found that the position of the protein has changed over several generations, transferring from the endoplasmic reticulum to the cell membrane during thermal adaptation

    .
    "The most surprising thing is that the conformation of the protein is also different

    .
    It also changes the interacting proteins

    .
    "

    By examining the interactions between proteins and related molecular functions, the researchers found that Fet3p produced at different temperatures has different functions in different cell compartments
    .
    Zhou said that thermal adaptation changes the folding and function of proteins, allowing a polypeptide to adopt multiple structures and moonlight functions according to its growth environment

    .
    "These results collectively show the plasticity of the proteome and reveal previously unknown strategies available to organisms facing long-term temperature challenges

    .
    " For simple organisms like yeast, alternative splicing is very limited.
    This kind of proteome plasticity, Or the selective folding of proteins induced by environmental conditions allows this organism to survive in a very wide range of harsh habitats

    .
    "

    Although an evolutionary coding strategy was discovered that allowed yeast to adapt to different temperatures, Zhou pointed out that flexibility cannot be assumed
    .
    "We know that plasticity has a limit-beyond a certain temperature, yeast will die

    .
    Our hope is that this work will make people work hard to learn from Mother Nature and understand how organisms are plastic by implementing their protein coding.
    To adapt to climate change

    .
    Some species have experienced many climate changes in the history of the earth, and their genome/proteome may have learned how to tolerate these changes

    .
    At the same time, many species are still new to climate change, and they are likely

    We are facing the danger of extinction because of the current global warming .
    We are very happy to contribute to urgent issues at the molecular level and welcome cooperation

    .
    "

    Zhou will continue to in-depth study the molecular details of cell internal changes during long-term temperature changes, and plan to incorporate simple animals into the exploration of protein plasticity
    .
    He will also study the effect of temperature changes on aging

    .

    ###

    Proteome plasticity in response to persistent environmental change

    DOI: 10.
    1016/j.
    molcel.
    2021.
    06.
    028


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