Monitoring regulations and use guide of super clean worktable

Super clean bench is a widely used equipment in microbiological testing laboratory It is necessary to establish monitoring procedures and use guidelines 1 Monitoring procedures: the corresponding monitoring procedures shall be formulated for the management of ultra clean worktable, the main contents of which are as follows: ① the filter used to regularly monitor the microbiological quality of the inner surface of ultra clean worktable and sterile isolation system and the air quality (particles and microorganisms) inside shall be able to ensure that the retention rate of particles greater than or equal to 0.3um is over 99.97% The laminar air velocity shall be sufficient to carry particles out of the work area Generally speaking, in the plane (work area) 15-30cm away from the downstream of the filter, the ideal laminar flow speed should be controlled between 0.36-0.54m/s; ② the integrity and laminar flow rate of the filter should be confirmed, and the integrity (leakage) inspection and filter efficiency (performance) test should be carried out on the site after the installation In the future, such tests shall be carried out regularly (usually less than once every half a year); ③ once the daily management finds that the equipment is unstable, or the environmental monitoring results are abnormal, or the deviation of microbiological test results is on the rise, the integrity inspection and performance test shall be carried out immediately This kind of examination is very important, which can effectively avoid the potential pollution risk to aseptic operation During aseptic operation, the microbiological quality of the air in the operation area and the surface of the operation platform shall be monitored at the same time During the experiment, the control test of yin and Yang shall be conducted at the same time 2 Use guide: the main contents of the use guide of laminar flow purification workbench are: the laminar flow purification workbench should be placed at a place far away from the door, and the purpose is to make the air flow around the equipment relatively stable, not to affect the operation area, and not to interfere with the laminar flow in the operation area After the equipment is opened, the operator shall first check whether the reading of the differential pressure indicator is normal Compared with the normal differential pressure, the small or large differential pressure indicates that the equipment is abnormal, so it is necessary to investigate immediately and take necessary corrective measures If the UV lamp is installed in the operation area, the UV lamp must be turned off before operation to complete all sterile operations To prevent the damage of ultraviolet to the operator and the possible damage to the microorganism in the sample Operators can only operate in laminar flow area after wearing sterile latex gloves and sterile sleeves After opening the worktable, the surface of the worktable shall be sterilized with sterile disinfectant (70% sterile ethanol), and the operation can be started after waiting for 5 minutes less Before aseptic operation, all articles placed in the workbench shall be fully sterilized and air dried All items in the working area shall be arranged in order to avoid disturbing the air flow direction The idle articles in the workbench shall be removed immediately, and the air flow inlet shall not be blocked Operators shall be familiar with the design, working principle and air flow mode of laminar flow purification bench The air flow Zui in the ordinary laminar flow purification bench is finally directly facing the operator and exhausting to the surrounding environment Therefore, this kind of purification bench shall not be used to deal with infectious substances, toxic substances or sensitive substances They are only suitable for general aseptic operation activities, such as assembly of aseptic articles, microbiological examination of conventional articles, preparation of culture medium, etc Although on the surface, the air flow in the working area of the vertical laminar flow purification bench will cause potential pollution to the operators and the surrounding environment without any personnel and surrounding environment; the operators should avoid pollution to the surrounding environment and operators When using the laminar flow table, make sure that the filter is not blocked, so as to ensure that the clean air blows to and covers the whole working area In order to avoid disturbing the laminar flow, the operator should avoid moving hands and arms in and out of the laminar flow area as much as possible Even if they move, they should be careful All activities should be carried out downstream (along the laminar flow direction) of the exposure area of sterile articles as much as possible The laboratory is equipped with a purification bench Zui, which is a biosafety cabinet, and is specially used for the treatment of conventional microbial strains, such as the identification of microorganisms, the preparation and cultivation of biological indicators, the preparation, preservation and use of standard strains for positive control or medium sensitivity test In the clean area, if conditions permit, try to keep the laminar flow purification bench in operation However, once the operation is stopped and restarted, the working area shall be thoroughly disinfected and the pre operation time shall be less than 5 minutes before use In addition, before and after each use, clean and disinfect the work surface Detailed standard operating procedures shall be formulated for the operation, cleaning, disinfection, calibration and maintenance of various indoor laminar flow worktables Provide use and maintenance records for each laminar flow table and HTY sterile isolation system The use records include: the use date and time, the state before and after the use of the instrument, the state of cleaning or disinfection, and the user's signature, etc.; the maintenance records include: fault description, maintenance situation, and the signatures of the maintenance personnel and the person in charge of the equipment, etc Periodically review the equipment cleaning or disinfection records and environmental monitoring records to evaluate the maintenance status of the work area in the laminar flow table Shanghai Sujing Industry Co., Ltd if you have any need, you can pass with us at any time Thank you very much for your support and look forward to cooperating with you Thank you & bestragard! Suing Jinghua