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    Home > Biochemistry News > Plant Extracts News > Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other Artemisia Plants

    Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other Artemisia Plants

    • Last Update: 2021-03-10
    • Source: Internet
    • Author: User
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    Some plants in the genus
    Artemisia
    have been used for medicinal purposes. Among them,
    Artemisia iwayomogi
    , commonly referred to as “Haninjin,” is one of the major medicinal materials used in traditional Korean medicine. By contrast,
    Artemisia capillaris
    and both
    Artemisia argyi
    and
    Artemisia princeps
    , referred to as “Injinho” and “Aeyup,” respectively, are used to treat diseases different from those for which “Haninjin” is prescribed. Therefore, the development of a reliable method to differentiate each
    Artemisia
    herb is necessary. We found that a random amplified polymorphic
    DNA
    (RAPD) method can be used to efficiently discriminate a few
    Artemisia
    plants from one another. To improve the reliability of RAPD amplification, we designed primer sets based on the nucleotide sequences of RAPD products to amplify a sequence-characterized amplified region (SCAR) marker of
    A. iwayomogi
    . In addition, we designed two other primer sets to amplify SCAR markers of “Aeyup” (
    A. argyi
    and
    A. princeps
    ) along with “Injinho” (
    A. capillaris
    ) and
    Artemisia japonica
    , which are also traded in Korean herbal markets. Using these three primer sets, we developed a multiplex
    PCR
    method concurrently not only to discriminate
    A. iwayomogi
    from other
    Artemisia
    plants, but also to identify
    Artemisia
    plants using a single PCR process.
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