Multispectral Imaging and Automated Laser Capture Microdissection of Human Cortical Neurons: A Quantitative Study of CXCR4 Expression
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Last Update: 2020-11-30
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Source: Internet
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Author: User
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Quantifying protein and RNA expression within specific cell populations in vivo is an essential step in unraveling the complexmechanisms of neurological disease. The challenges associated with studying human brain tissue are commonly compounded byvariations in postmortem interval, formalin fixation time, and tissue processing methods among others. The result is a samplepopulation that is inherently heterogeneous, implying the need for reliable protocols that are sensitive to low levels ofantigen while minimizing background and nonspecific staining. Here, we describe a single immunohistochemistry protocol onformalin-fixed, paraffin-embedded human cortex which can be adapted to (1) quantify the relative protein expression of thechemokine receptor, CXCR4, using multispectral image or (2) isolate neuronal RNA through automated laser capture microdissection.
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