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    Home > Food News > Food Articles > my country has made progress in the regulation of intracellular oxygen levels in microalgae

    my country has made progress in the regulation of intracellular oxygen levels in microalgae

    • Last Update: 2021-08-13
    • Source: Internet
    • Author: User
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    Our scientific research team has made progress in the regulation of oxygen levels in microalgae cells and the selection of high-yielding fucoxanthin species
    My scientific research team has made progress in the regulation of intracellular oxygen levels in microalgae cells and the selection of high-yielding fucoxanthin species.


    August 3, reporters from China Ke Xueyuan Qingdao Institute of Bioenergy and process (hereinafter referred to as "Qingdao of Energy") was informed that the researchers led by Li Fuli Engineering Research Group Molecular Microbiology, regulation and high levels of oxygen in the microalgal cells Progress has been made in the selection of fucoxanthin species
    .


    The work done by Li Fuli presided over by the Chinese Kexue Yuan to help build Levin Institute of Modern Physics University researcher, and gained the support of key national research and development program and the National Natural Science Foundation of China


    Academy of Sciences

    As one of the known photosynthetic organisms with the highest carbon fixation efficiency, microalgae can convert CO2 in the environment, inorganic carbon in water and small organic carbon into carbon sources for their own growth and reproduction through photosynthesis
    .


    At present, there are broad prospects in the production of bio-energy and natural products


    However, the large-scale cultivation process of microalgae, especially under outdoor high temperature and high light conditions, usually leads to excessive production of reactive oxygen species (ROS) and photorespiration in microalgae cells, resulting in a decrease in cell growth rate
    .


    Li Fuli said that as a single-cell photosynthetic organism, the oxygen release level of the cell itself is closely related to the cell state and environmental impact.


    The molecular microbial engineering research group led by Li Fuli, aiming at the regulation of intracellular oxygen levels in photosynthetic single-cell microalgae, transferred the exogenous Vitreoscilla hemoglobin gene (vgb) into the cells of Nannochloropsis.
    oceanica for the first time In
    .


    Vitreoscilla hemoglobin can bind oxygen molecules at high oxygen levels and release oxygen molecules at low oxygen levels


    Li Fuli reports, the team also around microalgae germplasm and production of natural products have done a lot of research, through early with China Kexue Yuan irradiation marine diatoms heavy ion Cooperation Institute of Modern Physics - Phaeodactylum (Phaeodactylum tricornutum ), a large number of mutant algae species were obtained to screen the natural product of marine diatoms-Fucoxanthin (Fucoxanthin)
    .


    Fucoxanthin is widely found in large seaweeds and diatoms, and has many benefits to human health, such as anti-diabetic, anti-obesity, anti-inflammatory and other physiological activities


    Li Fuli reports, the team also around microalgae germplasm and production of natural products have done a lot of research, through early with China Kexue Yuan irradiation marine diatoms heavy ion Cooperation Institute of Modern Physics - Phaeodactylum (Phaeodactylum tricornutum ), a large number of mutant algae species were obtained to screen the natural product of marine diatoms-Fucoxanthin (Fucoxanthin)
    .
    Fucoxanthin is widely found in large seaweeds and diatoms, and has many benefits to human health, such as anti-diabetic, anti-obesity, anti-inflammatory and other physiological activities
    .
    Phaeodactylum tricornutum is a model organism of diatom rich in fucoxanthin
    .
    In the previous research, in order to simplify the fucoxanthin detection method, Li Fuli's research team developed a spectrophotometric calculation method instead of the traditional high-performance liquid chromatography (HPLC) detection method, using this method within 3-5 minutes The detection and calculation of the fucoxanthin content in the sample can be completed, which greatly improves the research efficiency (Marine Drugs, 2018, 16, 33; doi:10.
    3390/md16010033)
    .
    Recently, flow cytometry has been used to conduct high-throughput screening studies on the fucoxanthin content of mutant strains, and 488 nm excitation light was introduced to analyze the emission fluorescence of Phaeodactylum tricornutum
    .
    A unique spectral peak was observed at 710 nm, and a linear correlation was found between the fucoxanthin content and the average fluorescence intensity there
    .
    Flow cytometry was used to screen the mutants with high fucoxanthin content produced by heavy ion radiation.
    After 20 days of culture, the fucoxanthin content of the sorted cells was 25.
    5% higher than that of the wild type
    .
    This work provides an efficient, rapid and high-throughput method to screen mutants with high fucoxanthin production
    .
    This work was recently published in Marine Drugs
    .
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