MYB3 transcription factors regulate the new molecular mechanism of phenylalanine synthesis.

Recently, the Institute of Biotechnology of the Chinese Academy of Agricultural Sciences has made new progress in the study of the biosynthesis control mechanism of plant secondary metabolites, and discovered a new molecular mechanism for the regulation of phenylalanine synthesis of MYB3 transcription factors.
the study was published online May 8 in the prestigious international journal Plant Physiology.
plants are able to synthesize a variety of secondary metabolites, including alkaloids, steroids, flavonoids, sulfur glucosides and so on.
These secondary metabolites play a very important role in the process of plant adaptation, they are involved in regulating plant quality, growth and development, biological and non-biological stress and other physiological and biochemical processes, and R2R3-MYB transcription factors play an important role in regulating the biosynthesis of phenylpropane secondary metabolism.
In the MYB transcription factor of the toned mustard, the myB3, MYB4, MYB7 and MYB32 transcription factors of subsector 4 have transcription inhibition activity because they contain EAR inhibitory base sequence, but the molecular mechanism of regulating gene expression has not been explained clearly. Earlier studies by the
research team found that MYB4, MYB7, MYB32 factors interacted with the cellular nucle membrane protein SAD2, and fixed-point mutation analysis found that the MYBs protein conservative base sequence GY/FDFLGL's Asp,D) played an important role in protein interactions, which allowed myBs protein to enter the nuclei to regulate gene expression, according to the journal Plant Journal.
On the basis of the previous period, further research found that the regulatory mechanism of MYB3 is completely different from THATB4, MYB7, MYB32, yeast double hybridization experiments found that MYB3 can interact with LNK protein family members LNK1 and LNK2, this interaction depends on the LNK protein C end of the conservative base sequence R1 and R2, fixed-point mutation found conservative base sequence R1 and Asp, D) in R2 plays an important role in interoperability, transcription activation detection and analysis proves that LNK1 and LNK2 regulate the inhibitor function of MYB3 through their N-end ENT domain, and the above research work reveals in depth the molecular mechanism of MYB inhibitors to regulate the biosynthesis of plant secondary metabolites, which provides an important theoretical basis for plant metabolic engineering and quality improvement.
the paper to biology as the first completion unit, Zhou Meiliang associate researcher as the first author, Zhou Meiliang associate researcher and Wu Yanmin researcher as co-communication author.
research was funded by the Ministry of Science and Technology, the National Natural Science Foundation of China and the Chinese Academy of Agricultural Sciences science and technology innovation project.
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