echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Active Ingredient News > Infection > N Engl J Med: Zhang Feng published his first NEJM paper and released an upgraded SHERLOCK system to speed up new crown detection.

    N Engl J Med: Zhang Feng published his first NEJM paper and released an upgraded SHERLOCK system to speed up new crown detection.

    • Last Update: 2020-10-02
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    On February 14, 2020, the crispsR gene editor Professor Zhang Feng's team released detailed operational documentation for detecting new coronavirus using CRISPR/Cas13-based SHERLOCK technology.
    in the documentation show that SHERLOCK technology is extremely sensitive to detect new coronavirus, with only 10-100 copies of the virus detected per microlith.
    , the method is simple to operate, requiring only purified nucleic acid molecular samples, and can be tested in less than an hour in three simple steps.
    March 30, 2020, Professor Zhang Zheng of Shenzhen Third People's Hospital (The Second Affiliated Hospital of Southern University of Science and Technology) led a team to publish a paper entitled: Clinicals of the recovered COVID-19 patients with re-detectable positive RNA test on the medical preprint platform medRxiv.
    the paper was published in the annal of Translational Medicine.
    the study first reported the clinical characteristics of Fuyang patients, and the team used highly sensitive SHERLOCK technology to conduct new crown tests, proving that the false negative problem of commercial nucleic acid testing may be a potential cause of Fuyang phenomenon.
    is also the first time THATLOCK technology has been used in real combat for new crown detection, the effect is bright.
    September 17, 2020, Zhang Feng's team published an article in the New England Journal of Medicine entitled: Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing, and Zhang Feng's team announced an upgraded version of STOPCovid.V2, a new coronavirus detection process based on SHELOCK technology.
    upgraded STOPCovid.V2 build.V2 built on its original foundation and further improved stopCovid.V2 detection sensitivity by adding RNA from the magnetic bead-rich sample during sample preparation.
    addition, version 2.0 streamlines the operation of bead-rich RNA, removing the ethanol cleaning and purgation process and allowing the entire RNA-riching process to take no more than 15 minutes.
    , the team conducted a double-blind test that tested positive for 202 new coronaviruses and 200 samples of new coronavirus-negative nasopharyngeal swabs, which showed that STOPCovid.V2 was 93.1% sensitive and 98.5% specific.
    and positive samples take only 15-45 minutes to get results.
    about SHELOCK technology CRISPR/Cas system is a bacteria and germ-specific immune system, is the evolutionary history of life, bacteria and viruses to fight the resulting immune weapon, used to resist viruses or exogenetic particles.
    When an exogenetic gene invades, the defense system's CRISPR sequence expresses the RNA that is identified by the invasive genome sequence, and then the CRISPR-related protein (Cas, a nucleic acid in-cut enzyme) cuts the exogenetic genomic DNA at the sequence recognition for defensive purposes.
    CRISPR technology was discovered in the early 1990s, and on February 15, 2013, Zhang Feng et al. successfully applied crispr/Cas9 systems to gene editing in mammalian and human cells, quickly becoming the most popular gene editing tool in the fields of human biology, agriculture, and microbiology.
    Depending on the number of sub-base members of the effect nuclease, crispsR/Cas systems can be divided into two categories: the nucleases of the first type of system consist of multiple sub-base, and the second type of system of particular concern, consisting of a single protein, including type II, V and VI based on Cas9, Cas12 and Cas13 effect proteins.
    of these, unlike most Cas proteins, the VI.type system's nuclease Cas13 is a RNA nuclease that relies on RNA targeting, a single-to-use cutting RNA that does not cut DNA, and has important application value in molecular diagnosis.
    many of the world's most common or deadly human pathogens are RNA viruses (e.g. Ebola, Zika, HIV, influenza, etc.), new coronavirus, also RNA viruses.
    2017, Zhang Feng published a paper in the journal Science inventing CRISPR/Cas13-based virus detection technology.
    Zhang Feng named the test technology SHERLOCK, the same name as detective Sherlock Holmes, which allows the cut RNA to form strips, form visually visible clues, and visually display them.
    SHERLOCK detection technology is more accurate than traditional real-time PCR detection, and the price and time required for testing are significantly reduced.
    October 10, 2019, Pardis Sabeti, Zhang Feng, and others published a research paper entitled Programmable Exhibition and Detection of RNA Viruses Using Cas13 in the cell sub-journal Molecular Cell.
    Parson Sabeti, Zhang Feng, etc. combined Cas13's antiviral activity with its diagnostic capabilities to build a powerful and fast programmable diagnostic and antiviral system called CARVER (Cas13-assisted virus expression and read-out restrictions) to detect and eliminate RNA-based viruses in human cells.
    system may be used in the future to diagnose and treat viral infections, including those caused by new and emerging viruses.
    : CARVER, Cas13-assisted restriction of viral expression and readout, and CARVER also means sculptor.
    these studies show that CRISPR/Cas13-based gene editing tools can be used not only for rapid virus detection and diagnosis, but also for the removal of viruses and the treatment of diseases caused by viruses.
    .
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.