Nankai University team develops new method to achieve targeted repair of vascular injury

A research team led by Nankai University recently published a paper titled "Targeted Repair of Vascular Injury by Adipose-Derived Stem Cells Modified with P-Selectin Binding Peptide" in Advanced Science.

Cardiovascular diseases, including coronary artery obstruction, have become the number one killer of human health

Adipose-derived stem cells (ADSC) have become the main source of cells for tissue engineering and regenerative medicine because they are easy to isolate and self-renew

DPP modification

In this study, the researchers combined P-selectin-binding peptide (PBP) with polyethylene glycol-conjugated phospholipid derivative (DMPE-PEG) through the hydrophobicity between DMPE-PEG and the phospholipid bilayer.

After identifying this DPP (DMPE-PEG-PBP) modification, they first evaluated the effect of the modification on the behavior of ADSCs

Targeting of DPP-ADSC

Next, the researchers evaluated the targeting ability of DPP-ADSC in vitro and in vivo

In vivo experiments, guidewire-mediated injury of rat femoral arteries induced luminal expression of P-selectin

DPP-ADSC repair arterial injury

Afterwards, the researchers injected 5 × 10 ⁻⁶ M DPP-ADSC into a rat femoral artery injury model by intravenous injection to evaluate its repair effect

In theory, the ideal way to repair damaged arteries is to restore their original physiological structure

When evaluating the repair mechanism of DPP-ADSC, they found that DPP-ADSC adheres to the surface of the damaged blood vessel, reduces the further adhesion of white blood cells and platelets, reduces the local inflammatory response, and effectively inhibits the intimal proliferation of the damaged blood vessel

Finally, the researchers also evaluated the targeting ability of DPP-ADSC in a human femoral artery balloon injury model

in conclusion

In summary, the research team successfully used DPP to modify the cell membrane surface of ADSCs, and fully confirmed that ADSCs modified with 5 × 10 ⁻⁶ M DPP have stronger targets for activated platelets, activated endothelial cells and damaged blood vessels.

Original Search

Yan H, Mi X, Midgley AC, Du X, Huang Z, Wei T, Liu R, Ma T, Zhi D, Zhu D, Wang T, Feng G, Zhao Y, Zhang W, He J, Zhu M, Kong D , Wang K.