Nat commun: the laboratory system can realize the regeneration of DNA and protein
-
Last Update: 2020-02-21
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
Synthetic biology research not only includes observing and describing life process, but also trying to imitate natural life process In a recent study Scientists at the Martins reed Max Planck Institute of biochemistry have created a system that can regenerate parts of its own DNA and protein components Hannes mutschler, head of the biomimetic systems research group at the Max Planck Institute of Biochemistry, and his team are working to simulate genome replication and protein synthesis through a "bottom-up" approach Now, researchers have successfully produced an in vitro system in which two processes can be performed simultaneously "Our system itself can regenerate a large part of the molecular components," mutschler explained To start the process, researchers need a "guide book" and a variety of molecular "machines" and nutrients Translated into biological terms, this means the need for DNA templates, which contain information to express the proteins that are produced Protein molecules used as catalysts, as well as nucleotides, the basic components of DNA, and amino acids, the basic components of proteins Specifically, the researchers optimized a DNA based, synthetic protein expression system in vitro Thanks to some improvements, in vitro expression systems are now able to synthesize proteins very efficiently "Unlike previous studies, our system is able to read and copy relatively long DNA sequences," explained Kai libicher, lead author of the study Scientists have assembled up to 11 circular DNA fragments into the adult genome This modular structure allows them to easily insert or remove certain DNA fragments The largest modular genome copied by researchers in the study consists of more than 116000 base pairs, which has reached a very simple cell genome length In addition to the polymerases that are important for DNA replication, the artificial genome contains other protein coding genes, such as 30 translation factors from E.coli To show that the new in vitro expression system can not only replicate DNA, but also produce its own translation factors, the researchers used mass spectrometry Using this method, they determined the amount of protein produced by the system In the future, scientists hope to expand the artificial genome through other DNA fragments They hope to work with colleagues at max synbio, a research network, to develop a closed system that can survive by adding nutrients and disposing of waste Such minimal units can then be used, for example, in biotechnology as machines for the production of natural substances or as building even more complex systems Source of information: repetitive genome from the laboratory
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.