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Microscopic images of hemocyanoin (right) and tobacco mosaic virus (left) obtained by iDPC-STEM technique (top) and structure (bottom).
The relevant research results are also introduced and classified
Cryo-EM technology is still a relatively new technology compared to X-ray crystallography, which has been used for decades, and it has a decisive advantage: protein building blocks can be observed in a state of rapid freezing in the natural environment without having to convert them into artificial crystals
So far, this method has been mainly used for materials research and has achieved very high resolution
Together with thermo Fisher Scientific's research partners, he used iDPC-STEM to map the protein structure at a resolution of 3.
"In contrast, today's cryo-electron microscopes are a bit
In traditional cryo-electron microscopy techniques, a sample takes thousands, sometimes tens of thousands or hundreds of thousands, of snapshots from many viewing directions
essay
Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution
