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On June 8, 2021, the Peking University-Tsinghua Life Sciences Joint Center and the Yi Chengqi research group of the School of Life Sciences of Peking University published a titled "Detect-seq reveals out-of-protospacer editing and target-strand editing by" in Nature Methods.
1.
The author captures the intermediate (dU) produced by the cytosine base editor CBE ADDIN EN.
Figure-1 Detect-seq technology roadmap
2.
Researchers transfected human embryonic kidney cells HEK293T and human breast cancer cells MCF-7 into BE4max ADDIN EN.
Figure-2 Cas-dependent off-target sites identified in three different sgRNA samples.
3.
Interestingly, the researchers also discovered two new Cas-dependent off-target edits based on the Detect-seq technology: out-of-protospacer edit and target-strand edit.
Figure-3 Modes of editing outside the sgRNA binding region and target strand editing
In summary, the Detect-seq technology provides a highly sensitive, highly specific, and non-biased method for detecting CBE off-target sites in cells in the field of base editing
Peking University-Tsinghua Life Sciences Joint Center and Professor Yi Chengqi from the School of Life Sciences of Peking University are the corresponding authors of this article; Dr.
Original link: https://
Postdoctoral Recruitment: Relying on the Peking University-Tsinghua Life Sciences Joint Center and the School of Life Sciences, Peking University, this laboratory uses chemical biology, single-cell omics, gene editing and other means to modify DNA and RNA modification omics technology, biological functions and their Research on regulatory mechanisms, with particular attention to the application of DNA/RNA biology in the diagnosis and treatment of human diseases
http://
references
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Thuronyi, BW, et al.
, Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol, 2019.
37 (9): p.
1070-1079.