Nature sub-news: Alcohol "reduced" longevity, curcumin to remedy!

DNA methylation was once thought to be a stable epigenetic mark, but it is now thought to be dynamic
.

In recent years , the identification of the dynamic characteristics of epigenetic markers has provided a basis for evaluating the impact of environment and specific compounds on the epigenome of organisms

DNA methylation was once thought to be a stable epigenetic mark, but it is now thought to be dynamic

Curcumin is the main compound isolated from the rhizome of the turmeric plant.

This study used a three-step screening method.
First, seven compounds were screened to observe the effect on longevity.
Finally, it was found that when curcumin and ethanol were co-administered, the shortened lifespan caused by ethanol intake was restored, and ethanol intake was determined.
The amount of DNA methylation changes in a specific region
.

This study used a three-step screening method.
First, seven compounds were screened to observe the effect on longevity.
Finally, it was found that when curcumin and ethanol were co-administered, the shortened lifespan caused by ethanol intake was restored, and ethanol intake was determined.
The amount of DNA methylation changes in a specific region
.

This study used a three-step screening method.

1.
The screening of food bioactive compounds reveals the impact on the survival of bees

1, 1,

This study selected 7 food bioactive compounds that are related to human exposure through food, nutritional supplements, drugs, and abuse
.

First study whether the intake of these seven compounds will affect the lifespan of bees

This study selected 7 food bioactive compounds that are related to human exposure through food, nutritional supplements, drugs, and abuse

Kaplan-Meier survival analysis data showed that treatment with isovaleric acid, valproic acid , cyanocobalamin (vitamin B12) and folic acid had a significant impact on survival

The role of folic acid and cyanocobalamin in insects has received little attention

 

According to previous reports, when the concentration is 2.
5% (v/v), ethanol has a negative impact on the lifespan of bees
.

This study observes that ethanol concentrations as low as 1% (v/v) will lead to a shortened lifespan, indicating that low concentrations of ethanol are more harmful than previously assumed

According to previous reports, when the concentration is 2.

It is worth noting that when fed with a feed containing high concentrations of curcumin (100µg/ml) and ethanol (1%, v/v), the lifespan of bees was significantly increased compared to ethanol alone ( Nethanol/ethanol & curcumin).

2.
Assess the impact of biologically active compounds in food on DNA methylation

2,

Although the methylated genome of honeybees is relatively sparse, ELISA-based methods have been successfully applied to detect changes in overall DNA methylation
.

This study did not observe any significant effect of any test compound on the DNA methylation level; however, when the bees were fed ethanol alone, a hypermethylation trend was indeed observed
.
It is worth noting that this trend has been reversed in bees that ate feed containing ethanol (1% (v/v)) and the highest curcumin concentration (100 μg/ml)
.

Although the methylated genome of honeybees is relatively sparse, ELISA-based methods have been successfully applied to detect changes in overall DNA methylation
.
This study did not observe any significant effect of any test compound on the level of DNA methylation; (1% (v/v))

3.
Curcumin blocks alcohol-related DNA methylation changes

Curcumin blocks alcohol-related DNA methylation changes

Compared with the control group, four areas of bees exposed to ethanol were methylated
.
Among them, three regions were hypermethylated, and one region was hypomethylated
.
Compared with pure ethanol-fed bees, curcumin and ethanol-fed bees have three regions with different hypomethylation
.
Further research found that curcumin and ethanol-fed bees had three methylation differences that overlapped with the three methylation differences observed in ethanol-fed bees compared with pure ethanol-fed bees
.
Ethanol induced hypermethylation in these three regions (Figure 3A-C), and curcumin had a significant protective effect on the hypermethylation of all regions induced by ethanol
.

Compared with the control group, four areas of bees exposed to ethanol were methylated
.
Among them, three regions were hypermethylated, and one region was hypomethylated
.
Compared with pure ethanol-fed bees, curcumin and ethanol-fed bees have three regions with different hypomethylation
.
There are three regions of different hypomethylation in curcumin and ethanol-fed bees
.
Further research found that curcumin and ethanol-fed bees had three methylation differences that overlapped with the three methylation differences observed in ethanol-fed bees compared with pure ethanol-fed bees
.
Ethanol induced hypermethylation in these three regions (Figure 3A-C), and curcumin had a significant protective effect on the hypermethylation of all regions induced by ethanol
.

The level of DNA methylation caused by the use of curcumin and ethanol was similar to the level observed in three-quarters of the ethanol-affected area of ​​control bees (Figure 3A-C)
.
The three ethanol-affected zones and curcumin blocking zones are located in the genomes of GB49219, GB47217 and GB43904, respectively, in linkage groups 7, 11 and 8, respectively
.
Among them, GB49219 is necessary for male reproduction
.
On this basis, it will be interesting to study whether drones will experience a brief decline in sperm quality after excessive drinking, as human males do
.
In an area of ​​ethanol-induced hypomethylation, curcumin failed to completely protect this hypomethylation, but a partial protection trend was observed (Figure 3D)
.
This region is part of the GB52826 gene body.
GB52826 is a transmembrane protein that seems to be widely expressed in human tissues and is associated with thyroid cancer
.

The level of DNA methylation caused by the use of curcumin and ethanol was similar to the level observed in three-quarters of the ethanol-affected area of ​​control bees (Figure 3A-C)
.
The three ethanol-affected zones and curcumin blocking zones are located in the genomes of GB49219, GB47217 and GB43904, respectively, in linkage groups 7, 11 and 8, respectively
.
Among them, GB49219 is necessary for male reproduction
.
On this basis, it will be interesting to study whether drones will experience a brief decline in sperm quality after excessive drinking, as human males do
.
In an area of ​​ethanol-induced hypomethylation, curcumin failed to completely protect this hypomethylation, but a partial protection trend was observed (Figure 3D)
.
This region is part of the GB52826 gene body.
GB52826 is a transmembrane protein that seems to be widely expressed in human tissues and is associated with thyroid cancer
.

In conclusion, the results of this study show that biologically active food ingredients can regulate the lifespan of bees
.
The negative impact of ethanol on longevity may be promoted by changes in DNA methylation, and when curcumin is consumed with ethanol, these changes can be prevented
.
This study shows how to restore longevity and DNA methylation changes through curcumin, a biologically active food ingredient
.
In addition, by introducing curcumin intervention, we use honeybees as a model system to study changes in DNA methylation associated with ethanol consumption
.

In conclusion, the results of this study show that biologically active food ingredients can regulate the lifespan of bees
.
The negative impact of ethanol on longevity may be promoted by changes in DNA methylation, and when curcumin is consumed with ethanol, these changes can be prevented
.
This study shows how to restore longevity and DNA methylation changes through curcumin, a biologically active food ingredient
.
In addition, we have introduced curcumin intervention.
In short, the results of this study show that biologically active food ingredients can regulate the lifespan of bees
.
The negative impact of ethanol on longevity may be promoted by changes in DNA methylation, and when curcumin is consumed with ethanol, these changes can be prevented
.
This study shows how to restore longevity and DNA methylation changes through curcumin, a biologically active food ingredient
.
In addition, we introduced curcumin intervention to prevent the use of honeybees as a model system for studying changes in DNA methylation associated with ethanol consumption
.

Original source:

Erik MK Rasmussen, et al.
Screening bioactive food compounds in honey bees suggests curcumin blocks alcohol-induced damage to longevity and DNA methylation.

Scientifc Reports | (2021) 11:19156 | https://doi.
org/10.
1038/s41598-021-98614-4

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