New cell adhesion analysis techniques monitor multiple cells simultaneously

analysis of multiple cell groups simultaneously with fluorescent markers is an efficient and reliable way to detect cell-to-cell interactions, speeding up this long and cumbersome and limited process. The new method, developed by researchers at King Abdullah University of Science and Technology, could also be used to study the process of inflammation or cancer cell metastasis, as well as to evaluate potential treatments.
cells move through the blood vessels that the cells adhere to - the special molecular interactions and attachments on the cell surface. In the blood stream, shear forces act on the cells to help coordinate cell adhesion. But this control can lead to inflammation and disease, such as cancer, while pathogens also use cell adhesion to infect hosts.
" traditional test for studying cell interactions is the Parallel Plate Flow Chamber (PPFC) test, which records cells rolling and clinging to endotrosaur cell molecules in the flow. Jasmeen Merzaban, who led the study, said, "This method of analysis has been used for decades, but it is prone to errors and deviations, and it can only analyze one cell type at a time, so it is time-consuming." Ayman Abu
, a graduate student at The Merzaban Labs in Washington, D.C., and colleagues hope to improve PPFC and speed up analysis.
new fluorescent multicellular rolling test (FMCR) proposed by the researchers uses unique fluorescent markers that can label up to seven cell types at the same time. Cell samples are mixed through a layer of endothor cells before entering the simulated flow. The researchers used a spectral confocus microscope to capture images of mixed cell clusters in real time. This allows researchers to collect cytodynamic data, including rolling frequency, speed, and the ability to bundle individual cell types.
"We have developed a comprehensive data analysis pipeline that allows us to analyze the various cell types obtained through this method and achieve high statistical power and sensitivity. "FMCR is now used to study the migration of a variety of human cells, including stem cells, active immune cells, and breast cancer cells.
advantage of this new method is that a compound, such as a new drug, can be added to cells before or during the trial to study the effects of the compound on cell adhesion.
" provides important insights into the effects of treatment on cell migration and the role of drugs in the body. Merzaban said.
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