New progress has been made in the study of the molecular mechanism of oil palm response to low temperature stress in the coconut plant of the Chinese Academy of Tropical Agricultural Sciences

New progress has been made in the study of the molecular mechanism of oil palm response to low temperature stress in the coconut plant of the Chinese Academy of Thermal Sciences, and the functions of MYB and AP2/ERF transcription factors have been extended to the regulation of low temperature response of oil palm for the first time, and the participation of MYB111 transcription factor in the regulation of low temperature stress response process of oil palm has been clarified
.
This study deeply analyzes the response mode of oil palm to low temperature stress, and lays a theoretical foundation
for the cultivation of new hardy oil palm varieties adapted to China's climate through molecular breeding.

 

As one of the typical tropical oil crops, oil palm likes high temperature, and China's hot area is located in the northern edge of the tropics, and low temperature has different degrees of impact on oil palm growth and yield, which is a key factor
limiting the expansion of oil palm planting area in China.
At present, the research on oil palm cold resistance mainly focuses on physiological and phenotypic evaluation, the molecular mechanism of oil palm cold tolerance is not clear, and the molecular mechanism of transcription factors involved in cold resistance response has rarely been reported
.
The research team completed the genome-wide identification of members of the oil palm MYB family, and applied bioinformatics methods to analyze their gene structure, conserved domains and gene collinearity, and analyzed their expression changes under low temperature stress by real-time PCR, and obtained a total of 159 MYB transcription factors in oil palm and found that 15 MYB genes were expressed
at ultra-high levels during low temperature stress 。 When the low temperature stress reached 24 h, the expression of EgMYB111 in oil palm was the most significant, and the EgMYB111 gene was transferred to Arabidopsis, and the results showed that the overexpression of this gene improved the cold resistance of transgenic Arabidopsis
.

 

The research results were published in Enviro under the title "Genome-wide identification and expression analysis of MYB gene family in oil palm (Elaeis guineensis Jacq.
) under abiotic stress conditions" nmental and Experimental Botany》，“The oil palm R2R3? MYB subfamily genes EgMYB111 and EgMYB157 improve multiple abiotic stress tolerance in transgenic Arabidopsis plants" in Plant Cell Reports
.

 

Zhou Lixia, assistant researcher of the Institute of Coconut, Chinese Academy of Thermal Sciences, is the first author, and the relevant research results are supported
by the Basic Research Funds of the Chinese Academy of Tropical Agricultural Sciences (1630152019001), the National Natural Science Foundation of China (31870670) and the Natural Science Foundation of Hainan Province (319QN324).

 

Related article links:

 

　　https://doi.
org/10.
1016/j.
envexpbot.
2020.
104245

 

　　https://doi.
org/10.
1007/s00299-021-02814-1