New progress in extraction of isoflavones from red clover in China

The research group of pharmaceutical chemical composition in the Key Laboratory of plant resources chemistry with northwest characteristics of Chinese Academy of Sciences has recently obtained the national invention patent license for the simple and efficient extraction of isoflavones from red clover According to reports, this has laid an important technical foundation for the development of health food and medicine products with isoflavones as the effective ingredients The laboratory is set up in Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences According to reports, isoflavone is a kind of health food and pharmaceutical raw materials In recent years, the international use of phytoestrogen like substances to replace hormone therapy, especially the use of natural sources of isoflavones in the treatment of women's menopausal syndrome, osteoporosis, Alzheimer's disease and other diseases has made great achievements Red clover is a kind of perennial herb of legume clover, which is a kind of high quality forage In our country, its inflorescence and branches and leaves with flowers are used as medicine to relieve spasm, cough and asthma It can also be made into ointment to treat local ulcer It is found that red clover is one of the few plants containing four kinds of estrogen like isoflavones, which has more than six times the estrogen like activity of most isoflavones, and has two-way regulatory effect At present, the extract of isoflavones from red clover is internationally recognized as a plant drug with definite therapeutic effect on women's menopause syndrome Its sales volume has been ranked in the top ten of plant drugs in the United States for many years, and China exports a large number of them every year Through one-step hydrolysis, water precipitation and alkali dissolution, membrane separation, column chromatography and other steps, the research group obtained a series of high-purity isoflavones On the one hand, the purity of isoflavone aglycone, the active component in the product, which plays a role in the efficacy, was improved; on the other hand, the sample solution before the macroporous adsorption resin column chromatography was purified, the damage of the resin was reduced, and the service cycle of the resin was prolonged In addition, the application of nanofiltration technology makes the sample solution before resin column chromatography do not need to be concentrated, and the sample can be directly loaded, which simplifies the process According to the introduction, the technology is simple and suitable for industrial production In the preparation process, only edible alcohol is used as the solvent, which has the advantages of safety, less pollution and low cost It lays an important technical foundation for the development of health food and medicine products with isoflavone as the effective component, and has high social and economic benefits.