On April 8, 2021, Beijing time, Cancer Cell published an online study by the Jackson Genomics Medical Laboratory on how extrachromosomal circular DNA (ecDNA) participates in the transcriptional regulation of cancer genomes.
In cancer genomes, structural variations usually lead to changes in the three-dimensional structure of the genome, which in turn also changes the transcriptional regulation mediated by the three-dimensional genome.
The study used the three-dimensional genomic technology ChIA-PET and ChIA-Drop to study the ecDNA-autosomal interaction map in glioblastoma and prostate cancer, and found that ecDNA and autosomal DNA generally interact with each other, and the interaction occurs.
The researchers used FISH experiments to verify the specificity of the interaction between ecDNA and its target region, and through in vitro circular DNA transformation experiments confirmed that ecDNA can indeed up-regulate the expression of autosomal genes.
Related paper information: org/10.
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