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nitric oxide ( NO ) ase-linked immunoanalysing
" box instruction manual
reagents for research purposes only < style" ">Purpose: This kit is used to determine dogs serum, plasma and related liquid samplesnitric oxide (NO)content.
experimental principle:
this kit applies double antibody sandwich measurement specimendogsnitric oxide ( NO )level. Color with purifieddogsnitric oxide ( NO )antibody package ismicroplate", made into solid-phase antibodies, into the packet of monoantigen micro-holes in turn addednitric oxide (NO ) <"">", then combined with HRP " <">no antibodies, forming antibodies - antigen - > < >"""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" < """ """ > color is displayed. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color and the nitric oxide ( NO " is positively related. Measure the absorbance < at 450nm wavelengths" (" OD " values) with an enzymatic standard meter, and calculate the "span style <" color in the sample using a standard curve: black;">" <"span style"color:black;"> nitric oxide ( NO " concentration.
kit consists :
< "1" cellpadding,"0" cellspacing, "0" class, "MsoNormalTable" style, "BORDER-RIGHT: medium none; BORDER-TOP: medium none; BORDER-LEFT: medium none; BORDER-BOTTOM: medium none; BORDER-COLLAPSE: collapse; >kit composition
48 < ">"
96
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