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In general, all protein-protein interactions in the cell form a PPI network

Now, bioengineers at the University of California San Diego have developed a technique that can reveal proton pump inhibitors in thousands of proteins in a single experiment

The researchers described the technology in the " Molecular Cell " magazine on August 3

"PROPER-seq can scan the sequence of 10,000 x 10,000 protein pairs in one experiment," said Kara Johnson, who recently graduated from the University of California, San Diego (UC San Diego) with a PhD in bioengineering and the first author of the paper

The core idea of ​​PROPER-seq is to attach a unique DNA sequence to each PPI, and then read these DNA sequence tags through next-generation sequencing

The laboratory will start the PROPER-seq protocol with the cells of interest and obtain the output as a list of identified PPIs

Zhong's team applied PROPER-seq to human embryonic kidney cells, T lymphocytes, and endothelial cells, and obtained 210,518 PPIs involving 8,635 proteins

The team verified the PPIs identified by PROPER-seq (called PROPER v1.

The team also verified four PPIs identified by PROPER-seq through experiments, and these PPIs have not been reported in the literature before

Their results show that PROPER v1.

The research team found that PROPER v1.

Looking to the future, the team hopes that PROPER-seq can help researchers screen many protein pairs and identify PPIs of interest

Kara L.