Non-steroidal anabolic hormone drug residue analysis pretreatment method-purification (2)

3) Molecular Imprinting Technology (MIT)

MIT refers to a polymer preparation technology that matches the template molecule in the spatial structure and binding site.
It is an interdisciplinary subject derived from polymer chemistry, biochemistry, and materials chemistry
.

MIT is to imitate the formation mechanism of antigen-antibody, forming highly cross-linked rigid polymer around imprinted molecule.

Ma Jinyu and others synthesized DES molecularly imprinted polymer (MIP) using DES as the template molecule, a-methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the crosslinking agent
.

The obtained white bulk polymer is crushed, sieved, and the polymer particles are settled with acetone to remove the fine powder to obtain polymer particles with a particle size of 35-45 μm

4) Immunoaffinity chromatography (IAC)

IAC is based on the specific and reversible immune binding reaction of antigen and antibody.
When the sample containing the test component passes through the IAC column, the immobilized antibody selectively binds to the test object, and other unidentified sample impurities are not hindered Ground flows out of the IAC column, after washing to remove impurities, the antigen-antibody complex is dissociated, the test substance is eluted, and the sample is purified
.

Its advantage lies in its high efficiency and high selective retention capacity for target compounds, and is especially suitable for purification and enrichment of trace components in complex samples

Zhang Qin et al.
used DES as a hapten to prepare monoclonal antibodies.
The cross-reaction rates of the antibodies against DES, HES, and DIS were 100%, 11.
36%, and 299.
54%, respectively , with IC ₅₀ of 42.
54ng/mL, 38.
20ng/mL, and 14.
20ng/mL
.

The fixed antibody concentration is about 5mg/mL and the gel is coupled with cyanogen bromide activated Sepharose 4B.

Zhang et al.
used IAC technology to purify ZER, TAL, ZAN and a-ZOL in cattle muscle
.

The ZER monoclonal antibody was coupled with cyanogen bromide activated Sepharose 4B (coupling rate was 96.

5) Solid phase micro extraction (SPME)

SPME utilizes the heterogeneous equilibrium of the analyte between the matrix and the extraction phase to diffuse and adsorb the analyte to the stationary phase coating on the surface of the quartz fiber.
After the adsorption equilibrium, it is combined with GC or HPLC for separation and determination.
Component to be tested
.

This technology does not require the use of column packing and organic solvents for elution, and integrates extraction, enrichment and analysis

Deng Aini et al.
established the SPME-HPLC method for the determination of DES in chicken tissue and milk powder
.

The extraction effect of two kinds of extraction head fiber coatings of polydimethylsiloxane (PDMS, 100μum) and polyacrylate (PA, 85μm) on DES were investigated respectively

Related links: Pretreatment method for analysis of non-steroidal anabolic hormone drug residues-purification (1)