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    Home > Biochemistry News > Biotechnology News > Note on the use, cleaning, classification and use of cell petri dishes.

    Note on the use, cleaning, classification and use of cell petri dishes.

    • Last Update: 2020-09-02
    • Source: Internet
    • Author: User
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    Petri dishes are generally made of glass or plastic and are commonly used experimental supplies for the culture of microorganisms or cells, usually glass can be used for plant materials, microbial culture and wall culture of animal cells.
    plastic may be polyethylene material, suitable for laboratory inoculation, line, separation of bacteria operation, can be used for plant material culture.
    petri dish fragile, in cleaning and use should be careful to take light, after use to clean in time, stored in a safe, fixed position.
    1, soaking: New or used glassware should first be soaked with water, softening and dissolving the attachment.
    New glassware must be used before the use of a simple brush with tap water, and then soaked with 5% hydrochloric acid overnight;
    2, brushing: the soaked glassware into detergent water, with a soft brush repeatedly brush.
    do not leave dead corners and prevent damage to the finish of the surface of the vessel.
    will be brushed clean glassware washed, dried, ready to soak in acid.
    3, . Acid immersion: Acid immersion is the above-mentioned vessel soaked in cleaning liquid, also known as acid, through the strong oxidation of the acid to remove the surface of the vessel may remain.
    acid should not be less than six hours, generally overnight or longer.
    careful when you put the utensils.
    4, rinse: after brushing and acid-soaked utensils must be fully rinsed with water, acid-soaked utensils are flushed clean, directly affecting the success or failure of cell culture.
    hand-washed after the acid-soaked utensils, each vessel at least repeatedly "water injection - empty" more than 15 times, and finally with heavy steaming water soaked 2-3 times, drying or drying after packaging spare.
    1, according to the use of petri dishes can be divided into cell petri dishes and bacterial petri dishes.
    2, according to the different manufacturing materials are divided into plastic petri dishes and glass petri dishes, but imported petri dishes and disposable petri dishes are mostly plastic materials.
    , depending on the size can usually be divided into diameter of 35mm, 60mm, 90mm.
    150mm Petri dish.
    4, according to the separation of different can be divided into 2 separate Petri dishes, 3 separate Petri dishes and so on.
    5, Petri dish material is basically divided into two categories, mainly plastic and glass, glass can be used for plant materials, microbial culture and animal cell wall culture may also be used.
    plastic may be polyethylene material, there is a one-time and multiple use, suitable for laboratory vaccination, demarcation, separation of bacteria operation, can be used for plant material culture.
    1, the operation of the petri dish cover may be adhered to water beads or bacteria, the back of the petri dish cover can avoid water beads or microorganisms fall on the petri dish.
    2, culture process, bacteria in the process of metabolic reproduction will produce some harmful to the growth and reproduction of bacteria metabolites, release heat and water discharge, if not reverse culture will have water droplets into the medium, affecting the growth of the bacteria.
    3, if the culture target is to collect bacterial metabolites, and metabolites are soluble in water, inverted culture may be convenient to collect.
    Culture will produce more water vapor in the petri dish, water vapor condensation on the lid of the dish will produce water droplets, if the petri dish is placed, water droplets will wash away the colonies, so that a large colony may be dispersed into a lot of small colonies, bacteria culture and counting are causing great trouble.
    if caused, the meth is on top, the dish is under the lid, and the water droplets do not drip on the bacteria.
    1, before use after cleaning and disinfection, petri dish cleaning or not has a greater impact on the work, can affect the acidity of the media, if there are certain chemicals, will inhibit the growth of bacteria.
    2. Newly purchased petri dishes should be rinsed with hot water and then soaked in a hydrochloric acid solution with a mass score of 1% or 2% for several hours, so that free alkaline substances are removed and then rinsed 2 times with distilled water.
    3, to culture bacteria, and then use high-pressure vapor (generally 6.8 x 10 of the 5 square Pa high-pressure vapor), 120 degrees C temperature of 30min sterilization, dry at room temperature, or with dry heat sterilization, is to place the petri dish in the oven, temperature control at about 120 degrees C to maintain 2h, can kill the bacteria's teeth.
    4, sterilized petri dishes can be inoculated for culture use.
    .
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