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Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers

 Last Update: 2021-01-05
 Source: Internet
 Author: User

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vesicles structure function

nmr lipid profile

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Nuclear magnetic resonance (
NMR
) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from γ and CHIF, two membrane proteins that function as regulatory subunits of the Na
+
- and K
+
-ATPase.

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