Oleanolic acid

Oleanolic acid

Oleanolic acid

[Synonyms] Angelica sinensis , Oleanol, CaryopHyllin, Asstrantiagenin C, Giganteumgenin C, Virgaureagenin B

【Structural formula】

[Molecular formula and molecular weight] C ₃₀ H ₄₈ O ₃ ; 456.

[Source] The dried and mature pulp of Cornus of ficinalis

【Properties】White powder

This product is insoluble in water, but soluble in methanol , ethanol , ether , acetone and chloroform

Melting point: The melting point is greater than 300°C

Optical rotation: [α]D ₂₀ +73.

【Purity Check】

TLC

1.

Developing agent chloroform- methanol (40:1)

Check the color development of 10% sulfuric acid ethanol solution, heating at 105℃, inspection under ultraviolet light (254nm, 365nm) and sunlight

2.

Developing agent cyclohexane-chloroform- ethyl acetate - formic acid (20:5:8:0.

Check the color development of 10% sulfuric acid ethanol solution, heating at 105℃, inspection under ultraviolet light (254nm, 365nm) and sunlight

High performance liquid chromatography

Column CapCell Pack C ₁₈ , 5um (4.

Mobile phase acetonitrile-0.

Detection wavelength 210nm

【Structural identification】203

3406, 2943, 2862, 1695, 1464, 1387, 1032, 997

EI-MS m/z: 456[M] ⁺ , 248, 207, 203, 189, 133

¹ H-NMR (C ₅ D ₅ N) δ: 0.

¹³ C-NMR (C ₅ D ₅ N) δ: 38.
6(C-1), 27.
3(C-2), 79.
1(C-3), 38.
8(C-4), 55.
4(C-5), 18.
4( C-6), 33.
1(C-7), 39.
4(C-8), 47.
8(C-9), 37.
2(C-10), 23.
6(C-11), 122.
7(C-12), 143.
6(C -13), 41.
7(C-14), 27.
8(C-15), 23.
1(C-16), 46.
6(C-17), 41.
2(C-18), 46.
0(C-19), 30.
7(C- 20), 33.
9(C-21), 32.
8(C-22), 28.
2(C-23), 15.
5(C-24), 15.
3(C-25), 17.
2(C-26), 25.
9(C-27) ), 183.
0(C-28), 32.
5(C-29), 23.
5(C-30)
.

【Storage】Dry and airtight
.