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    Home > Active Ingredient News > Immunology News > [Olink Black Technology] Cell's new work reveals the secret of diet-intestinal flora-the mystery of the human immune system

    [Olink Black Technology] Cell's new work reveals the secret of diet-intestinal flora-the mystery of the human immune system

    • Last Update: 2021-10-02
    • Source: Internet
    • Author: User
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    Introduction: The Stanford University team used Olink immuno-inflammatory proteomics analysis and intestinal flora metagenomics study to find that eating high-fiber foods can maintain the stability of the intestinal flora and affect the function of the flora, such as the up-regulation of the carbohydrate-active enzyme CAZyme.
    Causes individualized inflammation; while eating fermented foods can increase the diversity of intestinal flora and reduce the expression of inflammatory markers in the human immune system

    .

    Click to read the original text and contact the technical experts of the Olink proteomics platform.
    On August 5, 2021, the research group of Professor Justiin L.
    Sonnenburg, a scientist from the Stanford University School of Medicine, Microbiology and Immunology, published their latest research results: intestinal microbes Group-targeted diet can regulate the immune status of humans.
    In this study, through a 17-week randomized prospective study (n=18/arm), combining the microbiome and the host's immuno-inflammatory proteomics study, they It is concluded that the high-fermented food diet has steadily increased microbiological diversity and effectively reduced plasma inflammatory protein markers.
    The above-mentioned multi-omics research data and conclusions are relevant to the inflammatory epidemic diseases that exist in today’s industrial society, such as oneself.
    Further research and dietary guidance on immune diseases, cancer and metabolic diseases have very important exploratory significance and application value

    .

    Seeing this, the author decided to eat fermented bean curd
    .

    The integration of gut microbiome and human biology, especially immunoomics, suggests that intervention in gut microbes may be a powerful means to change human health
    .

    A diet that targets the gut microbiota and enhances, introduces, or eliminates specific functions or groups can prove to be a powerful way to realize the promise of precision medicine
    .

    Non-communicable chronic diseases (NCCDs), such as many autoimmune diseases and even some tumors, are often driven by chronic inflammation, and their incidence is rapidly increasing with the global industrialization process
    .

    Coincidentally, the gut microbiota has also changed significantly with industrialization
    .

    Given that multiple studies are known to indicate that the human microbiota can affect the inflammatory state, a key question is whether a diet targeting the gut microbiota can reduce systemic inflammation in healthy individuals? In order to answer this question, Stanford University scholars used dietary intervention methods, and at the same time longitudinally monitored the microbiome and immune status of healthy adults participating in the experiment (see the figure below for the experimental design)
    .

    (A) Sequence flow chart of registration, assignment, follow-up and analysis of participants
    .

    The side panel shows the number of participants in the high-fiber (Fi) and high-fermentation (Fe) diet groups collected on each platform
    .

    *2 participants were specifically asked to be assigned to the high-fiber group, rather than randomly assigned
    .

    (B) The 14-week study outlines the timeline, sample type collection and corresponding experimental platform
    .

    (C) The fiber intake of the high-fiber diet group shown in the box plot; the fiber intake of the high-fermented food diet group is represented by a dotted line
    .

    (D) Box chart: The intake of fermented food in the high-fermented food diet group; the intake of fermented food in the high-fiber diet group is the dotted line
    .

    The researchers used 16S RNA sequencing technology to detect the intestinal flora of the subjects.
    They found that the diet of high-fermented food increased the diversity of the microbiota and changed its microbial composition (see the figure below); Compared with the fiber diet group, the overall alpha diversity of the microbiota of the participants who ate the high-fermented food diet increased during the intervention process, which can be seen from the overall ASV and Shannon diversity index changes

    .

    This increase in diversity persisted during the selection period, when the intake of fermented food was higher than the baseline but lower than the end of the maintenance period, indicating that the increase in diversity may involve the restructuring of the gut ecosystem, rather than being reflected immediately Consumption
    .

    In addition, high-dimensional immune system atlas analysis revealed the host's immune response to different food intake
    .

    Scientists have found that the intake of fermented food reduces the level of inflammation
    .

    Using Olink's immuno-inflammatory proteomics product Inflammation Panel to detect circulating cytokines in serum, 92 immuno-inflammatory markers were identified
    .

    As shown in the figure below, the expression of 19 protein markers decreased in the fermented food intake group, including cytokines, chemokines and inflammatory serum proteins, such as NGF, LIF-R, IL-6, IL-10, IL -12b, VEGFA, MMP-10 CCL9, and the like
    .

    Through the use of LME to calculate the negative correlation of the cross-time level of each analyte, it is found that these 19 immuno-inflammatory markers (Fgf-21 also has significant changes, not shown in the figure), from baseline (3 weeks) to the end of the intervention ( The 10th week; SAM two types of pairings, FDR% 0.
    05, q value% 0.
    1) have significant changes

    .

    Note: NPX refers to the normalized protein expression used in the log2 scale of Olink Proteomics
    .

    This article shows us that the longitudinal analysis method of the microbiome and immuno-inflammatory proteome at the multi-omics system level can be used as a research method to study the coordination of the host-microbe relationship
    .

    Using multi-omics measurements of host parameters including the microbiome and Olink proteome, including the most advanced sequencing and Olink PEA immuno-inflammatory factor analysis technology, we found that high-fiber and high-fermented food intake affects the microbiome and Human biology
    .

    Olink's proteomic detection is characterized by high sensitivity, high repeatability, and high specificity, which provides researchers with excellent data quality, and has attracted more and more attention from researchers
    .

    Olink's immune response-related proteome detection kits are increasingly used in immune-inflammatory related research
    .

    Reference: Gut-microbiota-targeted diets modulate human immune status Wastyk, Hannah C.
    et al.
    Cell, Volume 184, Issue 16, 4137-4153.
    e14 More details about Olink Inflammation Panel This kit is related to inflammation-related cytokines, Involving biological processes such as inflammation, cytokine stimulation, and apoptosis.
    The Panel contains proteins that are mainly related to cancer, nervous system, inflammation, cardiovascular, pulmonary disease, and infectious diseases.
    As of press date, there have been 237 articles.
    Using the Inflammation Panel multiple cytokine detection platform, it is widely used in nervous system, cardiovascular, endocrine, inflammation, tumor, infection and immune diseases

    .

    Among these 237 articles, nearly half of them are closely related to population cohort studies and clinical trials, and have great potential for clinical translational applications
    .

    About Olink Proteomics (NASDAQ: OLK) Olink Proteomics was founded in Uppsala, Sweden, and is committed to breaking through the comprehensive bottleneck of protein detection in terms of "multiplicity/specificity", "sensitivity" and "detection throughput".
    , And then realize ultra-sensitive multiple protein marker detection, unbiased targeted proteomics and precise proteomics to help protein marker discovery, drug development, translational medicine, and make "multi-omics integration" truly feasible

    .

    Specifically, based on the patented PEA technology and adhering to the scientific spirit of rigor and transparency, we have developed and fully verified a series of groundbreaking Olink Panels
    .

    These panels can empower scientists to better understand real-time biology by accurately detecting 48-3072 biomarkers in 1-8μl of body fluid
    .

    Thus, discover innovative drug targets such as pQTL with the help of multi-omics; screen better disease prediction and prognostic markers; understand the MoA, Safety, PK, PD, Dose of drug effects; expand the indications of marketed drugs; develop companion diagnostics ; Accelerate the process of drug development and reduce the cost of drug development; at the same time, promote the transformation from clinical research to clinical application; improve the sensitivity and specificity of early screening methods for tumors and other diseases, and ultimately achieve precision medicine
    .

    Olink now covers 100% of the main signal pathways, realizing ultra-sensitive and unbiased targeted proteomics in the biological sense (compatible with various sample types, especially suitable for body fluid samples such as plasma, serum, etc.
    , which are incapable of traditional methods)

    .

    Official website: https:// Tel: +86 21 5077 8771 Email: China@olink.
    com Address: No.
    240, Hedan Road, Pudong New District, Shanghai Follow Olink China WeChat account to learn more
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