On August 17, 2021, Song Haiwei and Huang Jun's research group jointly published a paper on Cell Discovery to analyze the molecular and structural basis of INTS3/INTS6 protein complex involved in DNA damage repair

2021 Nian 8 Yue 17 days, hospital and Huang Jun Song Triton Task Force Task Force cooperation in Cell Discovery delivered a presentation entitled " the Crystal Structure of at The INTS3 / INTS6 Complex reveals at The Functional Importance of INTS3 dimerization in DSB Repair " research paper
.

This paper reveals the mechanism of INTS3 dimerization and the formation of INTS3/INTS6 heterodimers at the molecular level , and at the same time clarifies that the formation of this protein complex plays a vital role in the repair of DNA damage and the maintenance of genome stability.

Among all types of DNA damage, double-stranded breaks ( DNA double-stranded breaks, DSBs ) are the most threatening type of damage to cells
.

DSBs are mainly repaired through two methods: Non-homologous End Joining (NHEJ ) and Homologous Recombination (HR )

HR The first step is to repair breaks in double-stranded DNA for the 5 ' cut end, thereby generating 3' end of the ssDNA ( SINGLE-Strand DNA )
.

ssDNA protects it from nuclease attack by recruiting specific binding ssDNA protein, activates damage checkpoints and promotes HR repair

SOSS1 is a protein complex that specifically binds to ssDNA ( single-strand DNA ) cloned by Dr.
Jun Huang (Huang et al.
, Mol Cell, 2009 ) .
This complex plays a key role in HR repair by binding to long-chain ssDNA .
The SOSS1 complex contains three subunits:INTS3, SOSSC and hSSB1 , where INTS3 is connected and stabilized by the N- terminus as a scaffold protein .
However, the work (prior to our R & lt ENet Al.
, The Rep the Cell,, 2014 parsed) in SOSS1 structure (comprising INTS3 N- terminus, the full-length hSSB1 and full length SOSSC ) display, comprising only the I ntS3 N- terminus SOSS1 The complex can only bind to short-stranded ssDNA , soThe C- terminus of
INTS3 is also believed to play an important role .

It has recently been discovered that INTS3 C- terminus ( INTS3c ) can recruit INTS6 to participate in DNA double-strand break repair, but it is not clear how the INTS3/INTS6 complex affects the DNA damage repair process

 

INTS3c / INTS6c structure and facilitate SOSS1 complex binds to the long single stranded DNA schematic view of

 

Institute of Life Sciences, Zhejiang University, Dr.

Jia Yu, Dr.
number of days to repair and Singapore Technology Bureau of the molecular and cellular biochemistry postdoctoral SR Bharath is co-first author of the paper , Professor Song Hai and Professor Huang Jun Wei is a co-corresponding author of the paper

Original link: https://