One-Step One-Lane Chemical Sequencing of DNA
Last Update: 2021-03-01
Search more information of high quality chemicals, good prices and reliable suppliers, visit
There are several chemical methods for sequencing
. The classical method, introduced in 1977 by Maxam and Gilbert (
), uses four different chemical reactions to attack, modify, and open the heterocyclic bases at guanine (G), adenine and guanine (A + G), thymine and cytosine (T + C), and cytosine (C) in a radioactive end-labeled DNA fragment. The reactions result also in breakage of the glycosidic bond. A second reaction with hot piperidine is required to cleave the DNA backbone at these positions. The reaction products are thus analyzed by electrophoresis in four different lanes of a polyacrylamide gel. Several alternatives to the basic protocol were developed, as reviewed in (
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to email@example.com
. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.