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A generalized preparation protocol for open tubular (OT) molecular imprinted polymer (MIP) silica capillary columns for chiral separations in capillary electrochromatography (CEC) is described. For �different chiral molecules the same general composition of the MIP reaction mixture can be used except for the choice of the specific template. This protocol is valid for a variety of templates including acidic, basic, and neutral molecules, enabling formation of rugged and porous thin MIP layers on the inner surface of the capillary resulting in columns with high chiral separation efficiencies if the template has a good chiral recognition susceptibility.