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Optimization of analysis conditions for determination of 9 glucocorticoid residues in puffer fish, eel and grilled eel

 Last Update: 2021-09-20
 Source: Internet
 Author: User

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10.

(1) Optimization of extraction and purification conditions

Glucocorticoid drugs are fat-soluble hormones.

In the study of this method, experiments were mainly conducted on the purification effect and elution efficiency of the Cleanert Silica solid phase extraction column

(2) Optimization of chromatographic and mass spectrometry conditions

This method uses the 3200 Q TRAP model liquid chromatography-tandem mass spectrometer produced by AB Company for conditional experiments

In ESI(-) mode, glucocorticoid compounds form molecular ion peaks in the form of [M-H+HCOOH] ^- .

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