Orientation of the Agarose Matrix by Pulsed Electric Fields
-
Last Update: 2020-12-23
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
DNA
molecules less than ~20 kilobase (kb) pairs in size are efficiently fractionated by unidirectional electrophoresis in agarose gels (
1
). The variation of DNA mobility with gel concentration can be described by the Ogston mechanism of pore size distribution, if the observed mobilities are first extrapolated to zero electric field strength (
2
). Therefore, sieving of the macromolecules by the matrix appears to be the dominant mechanism of mol wt separation in this size range.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
