OsDER1: New factors for protein degradation related to rice endosomerinnetworks.

Rice seeds are storage organs of protein and starch.
in the adversity environment, the grouting seed will accumulate a large number of falsefolding and non-folding protein, resulting in internal network stress, which in turn affects the seed yield and quality.
previous studies have shown that the endosome-related protein degradation (ERAD) pathway plays an important role in removing misfolded proteins and the intramylastum network stress response.
Because the existing ER stress-inducing method is difficult to apply to seed system, the mechanism of ERAD in plant seeds is still very little known.
the Qu Leqing Research Group of the Institute of Plant Research of the Chinese Academy of Sciences found in the early research work that the specific suppression of the expression of small G protein SAR1 by the endosperm can cause the secretion protein to remain in the endosomen albee network, leading to the internal network coercion of rice seeds.
on this basis, the researchers isolated the endosomen network of rice seeds that were purified by internal matrix, and identified osDER1 as an endosconotetic force response factor through quantitative proteomic analysis.
researchers found that OsDER1 expression is induced by ER stress, excessive expression or inhibition of expression OsDER1 activates the response of unfolded protein, making rice more sensitive to endosomerline network stress, and significantly increased the level of ubiquitin; Further research
showed that OsDER1 interacted with the classic ERAD pathway core components OsHRD1, OsHRD3, and OsCDC48.
researchers also found that OsDER1 also interacts with the signaling peptide peptideoenzyme OsSPP.
recent studies have shown that SPP and DER1 can form a new ERAD pathway in yeast and mammalian systems, and the results of OsDER1's interaction with OsSPP suggest that this ERAD pathway is also conservative in plants.
this study helps to clarify the mechanism of plant ER stress response.
research was published on September 1st in the international academic journal Plant Physiology. Qian Dandan, an assistant researcher at
Qu Leqing Research Group, is the first author of the paper and Qu Leqing is the communication author.
the research was supported by the Key Research and Development Program of the Ministry of Science and Technology and the NSFC project.
Source: Plant Research Institute.