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    Home > Biochemistry News > Plant Extracts News > Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation

    Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation

    • Last Update: 2020-11-06
    • Source: Internet
    • Author: User
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    microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progresses through targeting protein-coding genes for mRNA cleavage or translation inhibition. Many miRNAs are also identified from cotton using computational and/or experimental approaches, including the next generation deep sequencing technology. However, the function of the majority of miRNAs are unclear. In this chapter, we describe a detailed method for overexpressing miR 156 in cotton using
    -mediated genetic transformation. This provides an approach to investigate the function and regulatory mechanism of miRNAs in cotton.
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