Oxidative stress and chronic inflammatory biomarker detection at Oxford Biomedical Research.

Reagents
Boxes - Leading the industry in best analytical methodsFor 29 years, Oxford Research (OBR) has been developing, producing and selling products that identify and analyze oxidative stress and chronic inflammatory biomarkers, leading the way in the best analytical methods and high product quality.. OBR other products: Xenoenic acid (peanut acid) metabolism,
cytokine
, signal transductivity, vascular biology, exogenetic metabolism biomarkers analysis kit, recombinant protein,
antibody
, protons, etc., thiodebic acid (TBARS)
quantitative detection of lipid peroxide. The OBR's improved approach reduces the background, is more accurate and reliable (10 nm compared to the color method sensitivity of 250 nm/fluorescence method), omits the steps that can produce biochromosic groups to affect the detection of boiling samples with strong acid, can be operated at room temperature, and saves time - only 15 minutes. Bio, health care or food samples can be detected.. 1. TBARS color testing kit (
microplate
), cargo number FR40, 96-well plate 2.Improved TBARS detection (bi cuvette), cargo number FR35,100 reactions3.TBARS fluorescence test kit (micro-hole plate), cargo number FR45, 96-well plateurine mixed or not mixed with 10 uM propylene dialehyde test results, this product is compared with the results of 2 other companies. Results obtained using OBR products are significantly less affected by interference than similar products in other companies.
, isostane is an excellent indicator for diagnosis and evaluation of oxidative stress.
the results obtained using isostane ELISA analysis kits from other companies have been criticized for not being well relevant to GC/MS results, while the data from OBR kits are highly relevant to the data obtained using instruments (see figure below).. 1. Urine ISO ELISA analysis kit (samples do not need to be drawn), cargo number EA85,96-well plate: detection of 15-isostane F2t levels in urine.. Isostane levels obtained using ORB's
ELISA kit
are highly relevant to GC/MS datapartial documentation:
(1) Morrow, J., Harris, T., Roberts, L., Anal. Biochem. 14:1-10 (1990).(2)
Morrow, J. D., Hill, K. Burke, R. F., Nammour, T.M., Badr, K. F., and Roberts, L.J., Proc. Natl. Acad. Sci. U.S.A. 87:9383-9387 (1990).(3)
Morrow, et. al., The Isoprostanes: Unique Bioactive Products of Lipid Peroxidation. Prog. Lipid Res. 36:1-21, 1997.(4)
Morrow, J., Awad, J.A., Boss, H.J., Blair, I.A., and Roberts, L.J., Proc. Natl Acad. Sci USA 89:10721-10725 (1992).(5)
Wang, et al., Immunological characterization of urinary 8-epi-prostaglandin F2a excretion in man. J. Pharm. Exp. Ther. 275:94-100 (1995). (6)
Morrow, J., Zackert, W., Yang, J., Kuhrts, E., Callewaert, D., Taber, D., Oates, J., Roberts, J., Quantity of the major urinary metabolite of the isoprostane 15-F2t-Isoprostane (8-iso-PGF2a) by a stable isotope dilute mass mass mass, Analytical Biochem. 269: 326-331 (1999). (7)
Roberts II, L.J., Morrow, J.D., Measurement of F2-isoprostanes as an index of oxidative stress in vivo, Free Radic. Biol. Med. 28:505-513 (2000).? . 2. 15-isostane F2t ELISA analysis kit (
serum
, plasma, urine and tissue), cargo number EA84,96-well plate: detection of serum, plasma, urine and tissue 15-isoestane F2t levels. Note: 15-Isoprostane F2t (15-Isoprostane F2t) Alias: 8-Table-Prosultin F2 alpha/8-Isoprostrantin F2 alpha/8-isoProstane (8-epi-PGF2 alpha/8-isoprostane) . Partial literature: (1)
Morrow, J., et al. (1990) Anal. Biochem. 14:1-10 (2)
Morrow, J. D., et al., (1990) Proc. Natl. Acad. Sci. 87:9383-9387 (3)
Morrow, et al., (1997) Prog. Lipid Res. 36:1-21 (4)
Morrow, J., et al., (1992) Proc. Natl. Acad. Sci. 89:10721-10725 (5)
Wang, et al., (1995) J. Pharm. Exp. Ther. 275:94-100 (6)
Morrow, J., et al., (1999) Anal. Biochem. 269:326-331 (7)
Roberts II, L.J., Morrow, J.D., (2000) Free Radical Biol. Med. 28:505-513 (8)
Morrow, J.D., Roberts II, L.J., Meth. Enz. 300: 3-12 3.  Creatine testing kit, cargo number CR01,96-well plate: mainly used for standardized human urine samples in the ad analysis, with other test kits used. . 4. Goat anti-15F2t-isoprane, purified IgG, multi-resistance, suitable for
immune histification
, enzyme-linked immunity, protein hybridization, cargo number IS20, 0.1 mg. . 5. Pre-treatment kit (urine pre-treatment) of glucosal glycosidease samples, cargo number GL85, 40 samples: used in support of ELISA kit. The average isostane in human urine is combined with glucosalic acid, and the range of glucose aldehyde acidification between individuals is very large, 28-80%. Therefore, urine samples are pre-treated with β-glucosalase, allowing the researchers to distinguish the differences in glucosal acidification between individuals and to detect isostane in the overall system. . The effect of glucoalde acidase treatment on F2-isostane concentration detection in urine samples was treated in the sample. After mixing urine samples from 6 people, they are divided into 2 parts. Partly mixed with 10 ng/ml synthetic 15-F2t-isostane. Data obtained from pre-treatment of samples with or without glucosal acidase.
, more oxidative stress research products: . 1. Total Antioxidant Capability Test Kit, no. TA02,96-well plate: An application note from Thermo Fiesel (AP-MIB-MSF01-0908) uses this product to compare the antioxidant capacity of beverage samples. This product uses reduced copper chelate and biochromic substances combined in 450nm to produce the principle of maximum absorption, the method is simple, sensitive, fast, the results and oxygen free agent absorption capacity (ORAC), water-soluble vitamin E equivalent antioxidant capacity (TEAC) and tricolor iron reduction antioxidant capacity (FRAP) method is highly relevant. . Part of the literature:
1.  Brook, R. (2008) Clin. Sci. (Lond). 115:175-187
2.  Cerutti, P. and Trump, B. (1991) Cancer Cell 3:1-7
3.  Cottone, S., et al.; (2008) J. Nephrol. 21:175-179
4.  Trachootham, D., et al.; (2008) Antioxid. Redox Signal. 10:1343-1374
5.  Frei, B., et al.; (1992) Molecular Biology of Free Radical System 23-45
6.  Ciencewicki, J., et al.; (2008) J. Allergy Clin. Immunol. 122:456-468?
7.  Kieth, M., et al.; (1998) J. Am. Coll. Cardio. 31:1352-1362
8.  Van-Zoeren-Grobben, et al.; (1997) Acta Pediatrics 86:1356-1362
9.  Allard, J.P., et al.; (1998) Am. J. Clin. Nutr. 67:143-147
10.  Santini S.A., et al.; (1997) Diabetes 46:1853-1858
11.  Fatokun, A., Stone, T. and Smith, R. (2008) Front. Biosci. 13:3288-3311
12.  Apak, R., et al.; (2004) J Agric Food Chem 52:7970-7981
13.  Apak, R., et al.; (2005) Free Radic Res 39:949-961
14.  Apak, R., et al.; (2007) 12:1496-1547?
15.  Apak, R., et al.; (2008) Microchim Acta 160:413-419?
16.  Apak, R., et al.; (2008) Anal. Chim. Acta 630:28-39 2.  Also prototype glutathione/oxidizing glutathione test kit, cargo number GT40,96-well plate: detection of oxidative stress Part of the literature: (1)
Tietze, F., (1969) Analytical Chemi.